Smad同源物1(Smad1)重组蛋白

Smad同源物1(Smad1)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Mothers Against Decapentaplegic

Homolog 1

Synonyms: Synonyms: Synonyms: Synonyms: Smad1, Mad1, Madh1

Species: Species: Species: Species: Rat

Size: 50µg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Nucleus.

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: His273~Ser468 (Accession # P97588),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 25kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 23.8kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 7.0

Rat Smad1

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-HWCSIVYY ELNNRVGERF HASSTSVLVD GFTDPSNNKN

RFCLGLLSNV NRNSTIENTR RHIGKGVHLY YVGGEVYAEC LSDSSIFVQS RNCNYHHGFH

PTTVCKIPSG CSLKIFNNQE FAQLLAQSVN HGFETEYELT KMCTIRMSFV KGWGAEYHRQ

DVTSTPCWIE IHLHGPLQWL DKVLTQMGSP HNPISSVS

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1.Bucelli R.C., et al. (2008) J. Pharmacol. Exp. Ther. 324:1172-1180.

2. Pangas S.A., et al. (2008) Mol. Cell. Biol. 28:248-257.

3. Li P., et al. (2007) Nephrology (Carlton) 12:466-473.

4. Fuller M.L., et al. (2007) Ann. Neurol. 62:288-300.

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