RalA 活化检测生化试剂盒

中文名称

RalA 活化检测生化试剂盒

英文名字

RalA Activation Assay Biochem Kit

供应商

Cytoskeleton

产品货号

BK040

产品报价

￥11049.792/KIT50assays

产品说明书

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购买方式

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产品新闻

背景资料

该RalA 活化检测生化试剂盒产品是Cytoskeleton品牌原装进口，Cytoskeleton品牌是细胞骨架研究专家，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。艾美捷作为中国区域的总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立专业技术支持团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述

收到该RalA 活化检测生化试剂盒RalA Activation Assay Biochem Kit产品后，查看厂家提供的产品说明书详细介绍。

产品特点

The Ras family of small GTPases consists of at least 35 related human proteins that include the oncoproteins HRAS, KRAS and NRAS as the founding members (Colicelli 2004). The Ral proteins, RalA and RalB, share 58% identity to these oncoproteins and 85% identity with each other (Chardin and Tavitian 1986). Ral proteins play an important role in diverse cellular processes including endocytosis, exocytosis, oncogenesis and the regulation of transcription and cell morphology (Feig 2003)Like other small GTPases, Ral proteins become activated when they switch from the GDP-bound state to the GTP-bound state (Takai, Sasaki and Matozaki 2001), and it is the GTP-bound form that specifically interacts with their downstream effector proteins. The fact that Ral family effector proteins will specifically recognize the GTP-bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Ral protein activation (Hofer, Berdeaux and Martin 1998). The assay uses the Ral Binding Domain (RBD) of the effector protein RalBP1. The RBD protein motif has been shown to bind specifically to the GTP-bound form of Ral. The fact that the RBD region of RalBP1 has a high affinity for GTP-Ral makes it an ideal tool for affinity purification of GTP-Ral from cell lysates. The RalBP1-RBD protein supplied in this kit has been expressed as an His-tagged fusion protein in E. coli. and bound to colored beads. This allows one to “pulldown” GTP-Ral complexed with Ral-BP1-RBD beads. This assay provides a simple means of analyzing cellular RalA activities in a variety of systems. The amount of activated RalA is determined by a Western blot using a RalA specific antibody.

保存建议

厂家推荐常温运输，收到产品后建议保存于4°C。

其他

Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。