双解丝蛋白1(TWF1)重组蛋白

双解丝蛋白1(TWF1)重组蛋白

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Twinfilin 1

Gene Names: TWF1

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: Actin-binding protein involved in motile and morphological processes. Inhibits

actin polymerization, likely by sequestering G-actin. By capping the barbed ends of

filaments, it also regulates motility. Seems to play an important role in clathrin-mediated

endocytosis and distribution of endocytic organelles

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Cytoplasm › cytoskeleton

Tissue Specificity: Specificity: Specificity: Specificity: Expressed at high levels in the colon, testis, ovary, prostate and

lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Ser252 (Accession # Q12792), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 30.26 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 30.26 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will

increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGS-MSHQTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD KDYDSFVLPL

LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE VFGTVKEDVS

LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVGVDT KHQTLQGVAF PISREAFQAL EKLNNRQLNY

VQLEIDIKNE IIILANTTNT ELKDLPKRIP KDSARYHFFL YKHSHEGDYL ES

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Beeler JF, et al.(1994) Mol Cell Biol 14 (2): 982-988.

2. Rush J., et al. (2005) Nat. Biotechnol. 23:94-101. 3. Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162. 4. Palmgren S, et al.(2002) J. Cell. Sci. 115 (Pt 5): 881-886.

5. Vartiainen MK, et al.(2003) J. Biol. Chem. 278 (36): 34347-34355.

6. Moseley,J.B. et al. (2006) J Cell Sci. 119 (Pt 8):1547-1557.

7. Hassel S, et al. (2004) Proteomics 4 (5): 1346-1358.

8. Beeler J.F., et al. (1994) Mol. Cell. Biol. 14:982-988.