表面活性剂Silwet L-77是拟喃芥、油菜、大肠杆菌等转化必须试剂之一，原产地为美国GE公司，GE原厂货号为SL77080596-10ml，SL77080596-35ml，我公司再提供10ML规格的分装产品S5505。
Silwet L-77说明书下载,请点击左边说明书
注意：近期市场上出现假冒SILWET L-77一般为NP40等表面活性物质，外观和SILWET L-77相似，但转化效率非常低，最高的转化效率也只有SILWET L-77的1/40，假冒SILWET L-77一般只能提供10ML分装产品，我公司有10ML原装和35ML原装，并可提供相关进口证明材料，我公司也可提供相关转化实验服务，费用请电话咨询。
Silwet L-77 is a chemical mixture of polyalkyleneoxide modified heptamethyltrisiloxane (84%) and allyloxypolyethyleneglycol methyl ether (16%). It is an essential infiltration media ingredient for all Agrobacterium-based transformations of Arabidopsis and other plants.10 mL and 35 mL are sufficient quantity to prepare 33 L and 115 L of vacuum infiltration medium when used at the recommended rate of 0.03%, v/v, respectively.
SILWET L-77 is a unique surfactant designed to improve the spreading and wetting properties of pesticide spray mixes. SILWET L-77 utilizes unique organosilicone chemistry which provides the most complete coverage possible. SILWET L-77 can improve cuticular penetration of plant surfaces by the spray mix. Optimum application and resultant effects can be influenced by many factors. Consequently, it is recommended that careful observation of the spray deposit be made and adjuvant rates adjusted accordingly. Applications should be made to ensure thorough coverage without excessive run-off.
TYPICAL PROPERTIES
Physical appearance Clear, light amber liquid
Pounds per gallon 8.370
Specific gravity 1.0 - 1.01
pH (1.0%) 6.0 - 7.0
Freeze Point less than 35 degrees F
Surface Tension (0.125% v/v) less than 25 dynes/cm
Contact Angle (0.125% v/v) less than 5 degrees F
Draves Wet Time (0.125% v/v) less than 15.0 seconds
GENERAL PRODUCT INFORMATION
(1) SILWET L-77 contains organosilicone surfactants designed to provide superior wetting and coverage.
(2) SILWET L-77 can improve water penetration of hard to wet soils and provide more uniform distribution of applied moisture.
(3) SILWET L-77 is generally compatible with pesticides and fertilizer solutions.
(4) SILWET L-77 may cause excessive foam if not properly added to the spray tank. To minimize foam generation, add SILWET L-77 to the tank after all other ingredients are added. If foaming is still a concern, add an antifoaming agent, such as FOAMBUSTER or COMBAT PLUS to the tank prior to adding SILWET L-77.
(5) Do not allow spray mixes containing SILWET L-77 to sit over 36 hours.
拟南芥的种植(拟南芥种植，拟南芥转化，油菜转化）
拌土：蛭石:黑土:珍珠岩=3:1:0.5用拟南芥PNS营养液浇，然后再将拟南芥种子点到土上。再用保鲜膜封住，再用报纸盖上数日后取走报纸，每天光照16小时。
拟南芥PNS营养液
1M磷酸缓冲液（PH5.5） 2.5ml
1M KNO3 5ml
1M MgSO4·7H2O 2ml
1M Ca(NO3)2·4 H2O 2ml
20mM Fe·EDTA 2.5ml
MS微量元素 1ml
加水定容至1L
