武汉艾美捷科技有限公司

微生物转氨酶重组蛋白/Recombinant Microbial Transglutaminase英文说明书：

Art. No.：T300

Synonym：EC 2.3.2.13; Protein-glutamine-γ-glutamyltransferase

Background info：AndraconTM is a brand for high quality microbial transglutaminase:

•Recombinantly produced in E. coli

•Ultra-pure and highly active

•Batch to batch consistent quality

•License free

•Produced according to an SOP in an ISO9001:2015 certified environment

AndraconTM (Microbial Transglutaminase, MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide-bound glutamine residues and a variety or primary amines.

Therefore, AndraconTM can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.

Essentially, AndraconTM is used for the production of antibody drug conjugates (ADCs).

This product replaces T001 and T153.

Molecular Weight：38334.0 Da (theoretical, activated MTG with N-terminal sequence: FRAPDSDDR...)

Source：Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.

Specific Activity：> 30 U/mg

Appearance：White lyophilized solid.

Formulation：The purified transglutaminase is lyophilized from 50 mM HEPES pH 7.4.

Reconstitution：Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.

Application：Labeling, immobilisation, conjugation, and modification of proteins.

Storage：Store at -80°C. Upon reconstitution, store working aliquots undiluted.

If storage at -80°C is not possible, storage at ≤ -20°C is recommended.

 

艾美捷微生物转氨酶重组蛋白/Recombinant Microbial Transglutaminase中文说明书：

产品编号：T300

同义词：EC 2.3.2.13; 蛋白谷氨酰胺γ-谷氨酰转移酶

背景信息：AndraconTM 是高品质微生物转谷氨酰胺酶的品牌：

•在大肠杆菌中重组生产

•超纯度高活性

•批间质量一致

•无需许可

在ISO9001:2015认证环境下，按照标准操作程序生产

AndraconTM（微生物转谷氨酰胺酶，MTG）催化肽结合谷氨酰胺残基的γ-酰胺基团与各种主要胺之间的酰基转移反应。因此，AndraconTM 可用于将具有主要胺的功能性标记物（如生物素、荧光染料、点击化学试剂或细胞毒素）附着到蛋白质上，例如抗体。

本质上，AndraconTM 用于生产抗体药物偶联物（ADCs）。

此产品替代了T001和T153。

分子量：38334.0 Da（理论值，激活的MTG具有N末端序列：FRAPDSDDR...）

来源：在大肠杆菌中重组生产。基因源自Streptomyces mobaraensis。

比活性：> 30 U/mg

外观：白色冻干固体。

配方：纯化的转谷氨酰胺酶从50 mM HEPES pH 7.4中冻干。

复溶：向冻干粉末的小瓶中加入与蛋白质冻干前相同的体积的水（见分析证书）。轻轻旋转小瓶直到固体溶解。

应用：蛋白质的标记、固定、偶联和修饰。

储存：在-80°C下储存。复溶后，储存未稀释的工作量分装。

如果无法在-80°C下储存，建议在≤ -20°C下储存。

 

Andracon微生物转氨酶重组蛋白/Recombinant Microbial Transglutaminase参考文献：

Kaempffe et al., J. Pharm. Sci. 2021, S0022-3549(21)00400-7;

Früh et al., ACS Nano 2021, 15, 12161–70;

Stricker et al., J. Pediatr. Gastroenterol. Nutr. 2019, 68:e43-e50;

Spycher et al., ChemBioChem 2017, 18:1923-7;

Steffen et al., J. Biol. Chem. 2017, 292:15622-35;

Dennler et al., Chembiochem. 2015, 16:861-7;

Dennler et al., Bioconjugate Chem. 2014, 25, 569-78;

Kaufmann et al., Food Addit. Contam. Part. A 2012, 29:1364-73;

Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49:9995-7;

Gianfrani et al., Gastroenterology 2007, 133:780-9;

Pfleiderer et al., Microbiol. Res. 2005, 160:265-71;

Ando et al., Agric. Biol. Chem. 1989, 53:2613-17;

Pasternack et al., Eur. J. Biochem. 1998, 257:570-6

 

仅用于研究用途，不用于人类、治疗或诊断应用。