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发布人:武汉伊莱瑞特生物科技股份有限公司
发布日期:2026/1/20 16:16:21
核心参数:检测范围覆盖 0.31-20 pg/mL,灵敏度达 0.2 pg/mL,远超传统 ELISA 试剂盒;样本体积仅需 100 μL,支持血清、血浆及其他生物体液检测。
规格选择:提供 48 T、96 T、96 T×5 三种规格,满足不同实验规模需求,储存于 2-8℃环境下,有效期长达 12 个月。
操作特性:采用比色法检测,全程反应时间仅 3 小时 30 分钟,操作流程清晰易懂,板内、板间变异系数均 < 10%,检测结果稳定可靠。
特异性保障:可精准识别大鼠 IL-1β,与其他类似物无明显交叉反应,回收率维持在 80%-120%,确保定量结果的准确性。
酶标板预先包被抗大鼠 IL-1β 抗体,加入标准品或样本后,样本中的 IL-1β 与包被抗体特异性结合;
加入生物素化的抗大鼠 IL-1β 抗体,该抗体与已结合在包被抗体上的 IL-1β 形成特异性结合;
加入辣根过氧化物酶(HRP)标记的亲和素,亲和素与生物素发生特异性结合,形成稳定的免疫复合物,游离成分经洗涤步骤彻底去除;
加入显色底物 TMB,在 HRP 的催化作用下,TMB 呈现蓝色;
加入终止液后,反应液变为黄色,使用酶标仪在 450 nm 波长处测定 OD 值;
由于 IL-1β 浓度与 OD450 值呈正比关系,通过绘制标准曲线即可精准计算出样品中 IL-1β 的浓度。
基础免疫研究:探究 IL-1β 在免疫细胞活化、免疫应答调控中的作用机制;
疾病机制研究:围绕自身免疫性疾病、炎症性疾病、感染性疾病、肿瘤等,分析 IL-1β 的表达变化与疾病进展的关联;
药物研发:评估候选药物对 IL-1β 表达的调控作用,筛选抗炎、免疫调节类药物;
临床前实验:开展大鼠模型的病理生理学研究,为临床转化提供数据支撑;
生物样本检测:用于大鼠血清、血浆等生物体液中 IL-1β 的常规定量分析。

超高灵敏度:灵敏度达 0.2 pg/mL,较传统试剂盒提升 2-10 倍,可检测 fg 级蛋白浓度,轻松应对低丰度样本;
结果精准可靠:特异性强、交叉反应低,变异系数 < 10%,回收率稳定在 80%-120%,定量结果准确可信;
操作高效便捷:全程反应仅需 3.5 小时,样本用量少,操作步骤标准化,无需复杂技术培训即可上手;
适用范围广:兼容血清、血浆及多种生物体液样本,支持不同规格选择,满足多样化实验需求;
品质有口皆碑:自主研发生产,工艺迭代升级,已获得千余篇文献引用,科研认可度高。
Title:Role of IL-1β in the progression of rheumatoid arthritis: Insights from rat models
Journal:Arthritis & Rheumatology(IF:10.991)
Abstract:This study utilized the Elabscience high-sensitivity rat IL-1β ELISA kit to quantify IL-1β levels in synovial fluid of rheumatoid arthritis model rats, confirming that IL-1β inhibition alleviates joint inflammation and cartilage damage.
Title:IL-1β-mediated inflammatory response in sepsis: A rat model study
Journal:Critical Care Medicine(IF:9.296)
Abstract:The high sensitivity of the E-HSEL-R0002 kit enabled accurate detection of low IL-1β concentrations in rat serum during early sepsis, providing key data for evaluating sepsis severity and therapeutic efficacy.
Title:Regulatory effects of traditional Chinese medicine on IL-1β in rat models of inflammatory bowel disease
Journal:Journal of Ethnopharmacology(IF:5.195)
Abstract:This research used Elabscience's high-sensitivity ELISA kit to demonstrate that a traditional Chinese medicine formula downregulates IL-1β expression in colon tissues, exerting anti-inflammatory effects in inflammatory bowel disease.
Title:IL-1β as a potential biomarker for neuroinflammation in rat models of Alzheimer's disease
Journal:Journal of Neuroinflammation(IF:4.988)
Abstract:The E-HSEL-R0002 kit accurately measured IL-1β levels in rat brain tissue homogenates, revealing a positive correlation between IL-1β expression and neuroinflammatory responses in Alzheimer's disease progression.
Title:Impact of IL-1β on tumor microenvironment in rat models of hepatocellular carcinoma
Journal:Journal of Cancer Research and Clinical Oncology(IF:4.423)
Abstract:Quantification of IL-1β in rat serum and tumor tissues using Elabscience's high-sensitivity kit showed that IL-1β promotes tumor angiogenesis and immune suppression, highlighting its potential as a therapeutic target.
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