| Name | Vacquinol-1 |
| Description | Vacquinol-1 is an MKK4 activator, which rapidly and selectively induces glioma cell death. |
| Cell Research | Cell viability is measured using CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer's instructions.(Only for Reference) |
| Kinase Assay | 1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 μM to wells containing the reaction mixture with tryptophan (0-200 μM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 μl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 μl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read[1]. |
| In vitro | Vacquinol-1 induces rapid and specific cell death of glioma cells with IC50 of 3.14 μM via membrane ruffling, cell rounding, massive macropinocytic vacuole accumulation, ATP depletion, and cytoplasmic membrane rupture. [1] |
| In vivo | In mice bearing U3013 mg tumors, Vacquinol-1 (15 μM intracranial injection, or 20 mg/kg p.o.) attenuates tumor growth and prolonging survival. [1] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 70 mg/mL (164.4 mM)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
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| Keywords | NSC 13316 | hepatocellular carcinoma | NSC-13316 | inhibit | Inhibitor | MKK4-specific activator | Vacquinol1 | induces apoptosis | glioblastoma | glioblastoma multiforme | HCC | activates MAPK pathways | Vacquinol-1 | NSC13316 |
| Inhibitors Related | JNK-IN-13 | Urolithin B | Astragaloside IV | SKLB-163 | SP600125 | (-)-Bornyl acetate | Anisomycin | SR-3306 | (E)-Ferulic acid methyl ester | Bentamapimod | Ginsenoside Re | TCS JNK 5a |
| Related Compound Libraries | Bioactive Compound Library | Pain-Related Compound Library | Kinase Inhibitor Library | Anti-Obesity Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library | Anti-Cancer Active Compound Library |
United States
