| Name | Tulipalin A |
| Description | Tulipalin A (α-methylene-γ-butyrolactone) (2-Methylenebutyrolactone) which has some phytotoxicity is a natural compound extracted from Tulipa gesneriana. |
| Cell Research | The influence of TUPA on cell viability was measured using the MTT assay. Cells were seeded in flat bottom 96-well plates at a density of 4 x 10^5 cells/ml. Afterward, Jurkat cells were exposed to TUPA in concentrations ranging from 10 to 82 μM. THP-1 cells (4 x 10^5 cells / ml) were exposed to concentrations ranging from 20 to 163 μM. After incubation for 72 h under humidified conditions (5 % CO2, 37 °C), 10 μl of MTT (5 mg/ml) per well were added and cells were further incubated for 3 h. Subsequently, 200 μl DMSO was added for cell lysis and formazan solubilization following incubation of the plates for 15 min while shaking. Afterward, absorbance was recorded at 550 nm and 620 nm using a microplate reader. In each plate, cells remained either untreated (negative control) or were exposed to Etoposide (2.5 μM) as a positive control. Using linear regression of the cell viability as a function of the compound concentrations, the concentrations leading to viability decreases of 10 % and 50 %, respectively, were calculated [2]. |
| In vitro | Tulipalin A-induced phytotoxicity is persistent allergic contact dermatitis documented in floral workers exposed to Alstroemeria and its cultivars [1]. THP-1 cells showed strong robustness to Tulipalin A treatment since only five proteins were altered. In contrast, in Jurkat T cells an increase in the abundance of 66 proteins and a decrease of six proteins was determined [2]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 45 mg/mL (458.71 mM)
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| Keywords | inhibit | Drug Metabolite | Allergic contact dermatitides,dermatitis | Tulipalin A | phytotoxicity TUPA | a-methylene-r-butyrolactone | Inhibitor | α-Methylene butyrolactone | alpha-methylene-gamma-butyrolactone |
| Inhibitors Related | Nortriptyline hydrochloride | Clofibric acid | Oseltamivir acid | Mycophenolate Mofetil | CLOZAPINE N-OXIDE | Monomethyl fumarate | Isonicotinic acid | Ampyrone | 4-Methylamino antipyrine | Nicotinamide N-oxide |
| Related Compound Libraries | 萜类天然产物库 | 经典已知活性库 | 植物来源化合物库 | 天然产物库 | 中药抗炎分子库 | NO PAINS 化合物库 | 高通量筛选天然产物库 | 活血化瘀中药单体库 | 已知活性化合物库 | 药食同源库 |
United States
