| Name | TOFA |
| Description | TOFA (MDL14514) is an allosteric inhibitor of acetyl-CoA carboxylase-α (ACCA). |
| Cell Research | NCI-H460, human lung cancer cells, and HCT-8 and HCT-15 cells (5,000/well) are seeded in 96-well plates overnight and then exposed to TOFA at indicated concentrations (0, 1, 5, 10, 20, 50 μg/mL) for 72 hours. Viable cells are detected using MTT assay[1]. |
| In vitro | TOFA (5-tetradecyloxy-2-furoic acid) inhibits cancer cell proliferation by inducing apoptosis, arresting cells in the G0/G1 phase, and showing time and dose-dependent cytotoxic effects. It targets Acetyl-CoA-carboxylase-α (ACCA), leading to reduced fatty acid synthesis, caspase activation, and cell death, particularly in prostate cancer (PCa) cells. TOFA exhibits significant cytotoxicity in lung cancer cells NCI-H460 and colon carcinoma cells HCT-8 and HCT-15, with IC50 values of around 5.0, 5.0, and 4.5 μg/mL, respectively. Concentrations from 1.0 to 20.0 μg/mL block fatty acid synthesis and induce cell death dose-dependently. It is also cytotoxic to COC1 and COC1/DDP cells, with IC50 values of approximately 26.1 and 11.6 μg/mL, respectively. |
| In vivo | The tumor growth rate is signifi?cantly inhibited by TOFA compared with the DMSO treated control mice (1649±356.3 vs. 5128±390.4 mm3. No toxicity is observed in the heart, liver, spleen, lung, kidney and intestinal tissues. TOFA inhibits COC1/DDP cell growth in ovarian tumor mouse xenografts. By inhibiting ACC, TOFA may be a promising small molecule agent for ovarian cancer therapy. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 6.88 mg/mL (21.19 mM)
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| Keywords | inhibit | RMI-14514 | Acetyl-CoA Carboxylase | ACC, Acetyl Coenzyme A Carboxylase | RMI 14514 | Inhibitor | MDL-14514 | TOFA | MDL 14514 |
| Inhibitors Related | ND-646 | Olumacostat Glasaretil | CMS-121 | PF-05175157 | Haloxyfop | CP-640186 hydrochloride | Firsocostat | PF-05221304 | CP-640186 | Quizalofop-P | Moiramide B | Tralkoxydim |
| Related Compound Libraries | Bioactive Compound Library | Anti-Obesity Compound Library | Inhibitor Library | Anti-Cardiovascular Disease Compound Library | NO PAINS Compound Library | Lipid Metabolism Compound Library | Metabolism Compound Library | Bioactive Compounds Library Max | Anti-Metabolism Disease Compound Library | Anti-Hypertension Compound Library |
United States
