| Name | TAPI-1 |
| Description | TAPI1 (TAPI) , an ADAM17/TACE inhibitor, inhibits shedding of cytokine receptors. |
| Cell Research | Five thousand cells are seeded into each well of 96-well plates and their viability is assessed by CellTiter-Glo Luminescent Cell Viability assay. Upon serum deprivation for 24 h, the cells are treated with Ang II for 60 h with and without pretreatment with each inhibitor and antagonist. The assay substrates are then added to each well on the plate and the samples are evaluated using a luminometer. (Only for Reference) |
| In vitro | TAPI-1 prevents unstimulated and PMA-induced release of the soluble forms of TNF-alpha, p60 TNFR, and IL-6R from the monocytic cell line, THP-1, and from human peripheral blood monocytes. TAPI also inhibits LPS-induced shedding of the p60 TNFR and TNF-alpha from monocytes. [1] TAPI-1 inhibits TACE-dependent constitutive release of co-transfected APP(695). [2] TAPI-1 attenuates Ang II-induced EGFR transactivation and cell proliferation in human HSC line LI90. [3] TAPI-1 with the EGFR inhibitor AG1478 exhibits deactivated AREG/EGFR/ERK signaling pathway and reduces pro-inflammatory cytokines release in pSS salivary gland-derived epithelial cells. [4] |
| Storage | store at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (6.61 mM), Sonication is recommended.
Ethanol : 92 mg/mL (184.15 mM), Sonication is recommended.
H2O : 55 mg/mL (110.09 mM), Sonication is recommended.
DMSO : 92 mg/mL (184.15 mM), Sonication is recommended.
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| Keywords | TAPI-1 Isomer | TAPI1 | MMP | Matrix metalloproteinases | Inhibitor | inhibit | cell surface proteins | ADAM17/TACE |
| Inhibitors Related | Stigmasterol | Doxycycline (hyclate) | Chondroitin sulfate | 3-Aminopropionitrile fumarate (2:1) | Astragaloside IV | Doxycycline | Edaravone | Glucosamine | Anethole | Meloxicam | Chloramphenicol | Tranexamic acid |
United States
