**R-Phycoerythrin: Definition, Functions, and Applications**
**Definition:**
R-Phycoerythrin (R-PE) is a red fluorescent pigment-protein complex derived from red algae. It belongs to the phycobiliprotein family and is widely recognized for its bright fluorescence and high quantum yield, making it a valuable tool in biotechnology and research.
**Functions:**
R-Phycoerythrin serves as a natural fluorescent marker due to its strong absorption and emission properties. It is highly stable and can be conjugated with antibodies, proteins, or other molecules, enabling its use in various detection and imaging applications. Its primary function is to enhance sensitivity and accuracy in fluorescence-based assays.
**Applications:**
1. **Flow Cytometry:** R-PE is commonly used as a fluorescent label for cell sorting and analysis.
2. **Immunoassays:** It is employed in ELISA, Western blotting, and other immunoassays for high-sensitivity detection.
3. **Microscopy:** R-PE is utilized in fluorescence microscopy for visualizing cellular structures and processes.
4. **Diagnostics:** It plays a key role in medical diagnostics, including cancer research and infectious disease detection.
5. **Biotechnology:** R-PE is used in protein-protein interaction studies and molecular labeling.
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