Description of PyBOP from Watson Biotechnology Co., Ltd
Basic Information of PyBOP |
Product Name | PyBOP |
CAS NO. | 128625-52-5 |
Apperance | White powder |
Assay | 99% |
Pacakge | 1kg/Foil Bag,25kg/Drum/Box |
MOQ | 1kg |
Storage | Cool and dry place |
Shelf Life | 2 Years |
Brand | Watson |
Specifications(COA) of PyBOP CAS 128625-52-5 Coupling Reagent
ITEMS | SPECIFICATIONS | RESULTS |
Appearance | White to off-white crystalline powder | White crystalline powder |
Identification by HPLC | The retention time of the main peak of the sample solution is consistent with that of the reference solution | Confirm |
Purity(HPLC) | ≥99.0% | 99.70% |
substances(HPLC) | HOBT≤1.0% | 0.13% |
substances(HPLC) | PyClOP≤0.2% | Not Detected |
substances(HPLC) | Related substances 4≤0.1% | Not Detected |
Maximum unknown single impurity | ≤0.3% | Not Detected |
Total impurities | ≤1.0% | 0.3% |
Melting point | 150.0-158.0℃ (dec.) | 153.8℃ |
Loss on drying | ≤0.5% | 0.10% |
Residual solvents | Ethyl acetate≤350ppm | 195.04ppm |
Conclusion | QUALIFIED. |
**PyBOP (Benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate)** is a coupling reagent widely used in organic chemistry, particularly in **peptide synthesis**. It facilitates the formation of **amide bonds** by activating carboxyl groups to form amides from amines and carboxylic acids. Here's a more detailed overview of its applications:
### Key Applications:
1. **Peptide Bond Formation**:
- PyBOP is primarily used for **coupling amino acids** during the synthesis of peptides. It converts the carboxyl group of one amino acid into a highly reactive intermediate, allowing it to couple with the amine group of another amino acid to form a peptide bond.
2. **Milder Reaction Conditions**:
- Compared to other coupling reagents like DCC (Dicyclohexylcarbodiimide), PyBOP works under **milder conditions** and tends to produce fewer side reactions, such as racemization (which is undesirable in peptide synthesis).
3. **Improved Solubility**:
- PyBOP is soluble in a variety of organic solvents, which enhances its use in solution-phase peptide synthesis.
4. **Less Formation of By-products**:
- It generates fewer by-products compared to other coupling agents like DCC, which produces urea as a by-product.
### Mechanism:
In peptide synthesis, PyBOP forms an active **Oxyphosphonium intermediate** by reacting with the carboxylic acid group of the amino acid. This intermediate can then react with an amine to form the desired amide bond (peptide bond).
In summary, PyBOP is essential in organic and peptide chemistry for efficient, high-yield peptide bond formation with minimal side reactions.
FAQ From Watson Biotechnology Co., Ltd |
Q | Can I get a sample for free ? |
A | For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test. |
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Q | How to confirm the product quality before placing orders ? |
A | Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order. |
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Q | What is the payment you accept? |
A | For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us. |
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Q | What is your shipment way ? |
A | For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement. |
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Q | How long is your after-sales service? |
A | We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company. |
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Q | How do you treat quality complaint ? |
A | We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers. |
Founded in 2006 and headquartered in Wuhan, Hubei, China, Watson Biotechnology Co., Ltd is a leading manufacturing powerhouse specializing in the production of Active Pharmaceutical Ingredients (APIs) and Intermediates, Food and Feed Additives, Agricultural and Aquaculture chemicals, and Fine Chemicals. As a dedicated factory, our operations are driven by a commitment to excellence and innovation, ensuring that our products meet the highest standards of quality and compliance.
With a robust team of over 100 skilled professionals, Watson Biotechnology has established itself as a trusted name in the industry. Our products are meticulously crafted to align with international standards such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), and BP (British Pharmacopoeia), guaranteeing reliability and efficacy.
At Watson Biotechnology, we pride ourselves on our cutting-edge facilities and state-of-the-art technology, which enable us to deliver superior products that cater to a diverse range of needs across various sectors. Our dedication to continuous improvement and customer satisfaction drives us to maintain stringent quality control processes and foster an environment of innovation and growth.
Watson Biotechnology Co., Ltd is a professional manufacturer which provide PyBOP with good quality and best price, welcome to contact us to get COA/TDS/MSDS of PyBOP.
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