| Name | PTP1B-IN-2 |
| Description | PTP1B-IN-2 is an effective protein tyrosine phosphatase 1B (PTP1B) inhibitor(IC50=50 nM). |
| Kinase Assay | The PTP1B enzymatic assay, the total volume of 100 μL per well contains15 nM recombinant PTP1B protein, 2 mM p-nitrophenylphosphonic acid (pNPP), 1 mM dithiothreitol and 1 mM EDTA (pH 6.5). After 30 min incubation at 37℃, end the reaction by addition of 2.5 M NaOH. The hydrolysis product, pNP, is detected at the absorbance at 405 nm. |
| In vitro | PTP1B-IN-2 extends deep into the active site pocket, forming several hydrogen bonds and hydrophobic interactions with key residues of the catalytic site. PTP1B-IN-2 greatly enhances insulin-mediated IRβ phosphorylation at concentrations of 15 μM and 30 μM. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 45 mg/mL (66.1 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (2.94 mM), Sonication is recommended.
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| Keywords | PTP1B-IN-2 | PTP-1B-IN-2 | PTP1BIN2 | PTP1B IN 2 | PTP1B | Phosphatase | Inhibitor | inhibit |
| Inhibitors Related | PTP1B-IN-22 | Disodium monofluorophosphate | β-Glycerophosphate disodium salt pentahydrate | Idoxuridine | Tartaric acid disodium dihydrate | Cyclosporine | Stearic acid | Cis-5-Norbornene-exo-2,3-dicarboxylic Anhydride | CaMKP Inhibitor | L-Ascorbic acid 2-phosphate magnesium | Cyclosporin A | Trimyristin |
| Related Compound Libraries | Glycometabolism Compound Library | Anti-Neurodegenerative Disease Compound Library | Bioactive Compound Library | Inhibitor Library | NO PAINS Compound Library | Metabolism Compound Library | Bioactive Compounds Library Max | Anti-COVID-19 Compound Library | Anti-Metabolism Disease Compound Library | Phosphatase Inhibitor Library |