Protein A (Staphylococcus aureus strain Cowan I) NEW

-Product Introduction

This product is a recombinant protein A expressed in Escherichia coli, purified by chromatography and freeze-dried to obtain a freeze-dried powder of recombinant alkali resistant protein A. This product is a modification of the protein structure based on natural Staphylococcus aureus protein A. It is composed of four Fc binding domain fragments linked together, with a molecular weight of 28KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant staphylococcus aureus protein A can be bound to agarose gel to prepare an affinity filler for antibody purification, which can separate and purify antibodies of various mammalian subtypes or genetic engineering recombinant proteins containing antibody Fc fragments from ascites, serum, cell culture supernatant or cell extracts. The C-terminal of Jibaikang recombinant alkali resistant protein A contains a cysteine, which can enhance its directional covalent bonding with gel.

-Product advantages

#High activity and stability: It exhibits good activity and stability in environments with high pH values, and can effectively hydrolyze phosphate compounds.

#High safety: As it is extracted and produced from human genes, recombinant alkali resistant protein A is non-toxic or immunogenic to humans, making it safer to use.

#Strong customizability: This product can be modified through genetic engineering technology to optimize its performance in specific environments and meet different application needs.

#Easy to produce: Through recombinant DNA technology, large-scale production can be carried out in the laboratory, ensuring sufficient supply of the product.

-Recommended usage method

This product is recombinant expressed in Escherichia coli, and its C-terminus is designed with a cysteine residue. It can be used to prepare affinity chromatography fillers through coupling reactions between thiol groups and epoxy groups on it. The activation of epoxide and protein A coupling methods need to be determined through experiments.

**Usage concentration: When using this product, it should be prepared according to the recommended concentration to ensure the best usage effect. Excessive concentration may cause non-specific interference, while insufficient concentration may not achieve the expected effect.

-Product Usage

#Immunoassay: Used to eliminate non-specific binding in immunoassays and improve the specificity and sensitivity of detection.

#Nucleic acid testing: During the extraction and purification of DNA and RNA, phosphate groups can be removed to reduce background interference.

#Proteomic research: used to eliminate phosphate compounds in samples, improve the accuracy and reliability of mass spectrometry analysis.

#Biosensors: Used to improve the specific recognition ability of biosensor surfaces.

-Transportation and storage stability

#Transportation stability: Ice pack insulation transportation, stable activity.

#Storage and transportation: It should be stored in a low-temperature refrigerator at -20 ° C to avoid repeated freezing and thawing. Appropriate insulation measures should be taken during transportation to prevent product failure due to excessive temperature.

-Notes and Disclaimer

#Try to use immediately after dissolution.   

#Avoid using reagents that can cause protein denaturation, such as strong acids, strong bases, strong oxidants, and high concentrations of organic solvents.   

#Avoid contamination: Recombinant alkali resistant protein A is sensitive to various microorganisms and endotoxins, so contamination should be avoided during use. All utensils and workstations that come into contact with the product should be properly disinfected.