Technical Data Sheet (TDS)
Product Name: Polyphenol Oxidase
Synonyms: PPO, Catechol Oxidase, Tyrosinase
CAS No.: 9002-10-2 (approx.)
Batch No.: PPO20251013
Enzyme Activity: ≥1000 U/g
Molecular Weight: 50–100 kDa (varies by source)
1. Product Description
Polyphenol Oxidase (PPO) is an oxidoreductase enzyme that catalyzes the oxidation of phenolic compounds to quinones, which subsequently polymerize to form brown pigments. It is widely used in food processing, biotechnology, and industrial applications.
Food Grade: Helps in flavor development, color formation, and bioactive compound modification in foods.
Industrial Grade: Used in biosensors, wastewater treatment, phenolic compound degradation, and enzymatic synthesis.
2. Physical and Chemical Properties
| Parameter | Specification / Description |
|---|
| Appearance | Light brown to brown powder |
| Odor & Taste | Characteristic |
| Solubility | Soluble in water, insoluble in organic solvents |
| Enzyme Activity | ≥ 1000 U/g |
| pH Range | 5.0 – 8.0 (optimum ~6.5) |
| Temperature Range | Stable: 4–30°C; Denatures > 50°C |
| Stability | Sensitive to heat, light, and extreme pH conditions |
3. Applications
Food & Nutraceutical Grade
Catalyzes browning reactions in fruits and vegetables for flavor and color development.
Used in enzymatic modification of polyphenols to improve antioxidant activity.
Applied in functional foods and supplements for phenolic biotransformation.
Industrial Grade
Bioremediation of phenolic wastewater.
Biosensors for phenolic compound detection.
Enzymatic synthesis of quinones and other industrial intermediates.
4. Quality Specifications
| Test Item | Specification |
|---|
| Assay (Enzyme Activity, U/g) | ≥ 1000 U/g |
| Loss on Drying | ≤ 5.0% |
| Heavy Metals | ≤ 10 ppm |
| Lead (Pb) | ≤ 2 ppm |
| Arsenic (As) | ≤ 2 ppm |
| Cadmium (Cd) | ≤ 1 ppm |
| Mercury (Hg) | ≤ 0.1 ppm |
| Total Plate Count | ≤ 1000 cfu/g |
| Yeast & Mold | ≤ 100 cfu/g |
| E. coli | Negative |
| Salmonella | Negative |
| Staphylococcus aureus | Negative |
5. Packaging and Storage
Packaging: 1 kg/aluminum foil bag or 25 kg/fiber drum, or as per customer requirement.
Storage: Store in a cool, dry place, protected from moisture and light.
Shelf Life: 12–24 months under recommended storage conditions.
6. Handling & Safety
Avoid inhalation of powders; use gloves and protective equipment when handling.
Enzyme is non-toxic at recommended food and industrial use levels.
Refer to MSDS (Material Safety Data Sheet) for detailed safety information.
7. Regulatory Information
Food Grade: Compliant with FDA and EU regulations for food enzymes.
Industrial Grade: Suitable for cosmetic, research, and industrial applications.
Not classified as hazardous under normal handling and storage conditions.
8. Additional Information
Origin: Plant or microbial-derived sources (depending on supplier).
Functionality: Catalyzes oxidation of polyphenols, enzymatic browning, biotransformation of phenolics.
Recommended pH and temperature: pH 5–8, 4–30°C for optimal stability
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