NADPH Tetrasodium salt
Empirical Formula (Hill Notation): C21H26N7Na4O17P3 · xH2O
Synonym(s): β-NADPH, Coenzyme II reduced tetrasodium salt, 2′-NADPH hydrate, β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, Triphosphopyridine nucleotide reduced tetrasodium salt, NADPH Na4, TPNH2 Na4, Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt
CAS Number: 2646-71-1
Molecular Weight: 833.35 (anhydrous basis)
Assay ≥97% (dry weight)
Form powder
Packaging pkg of 1 g
pkg of 100 mg
pkg of 500 mg
λmax 260 and 340 nm
Shipped in ice bag
Storage temp. 2-8°C
DESCRIPTION
General description
Amorphous powder bottled under nitrogen.
Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm
Absorbance maxima: 260 nm and 340 nm
Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4
pH value in water: 8.0 ± 1.0 (10 mg/ml)
Application
NADPH-RO has been used for the determination of thioredoxin reductase activity.
Biochem/physiol Actions
NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.
Quality
Purity: =97% ß-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm)
Organic solvents: =3% (GC)
Preparation Note
Storage conditions (working solution): 2 to 8 °C
10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.
Analysis Note
Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm
Other Notes
Packaged based on solid weight.
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