| Name | N1-Methylpseudouridine |
| Description | N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA. |
| In vitro | In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA .?Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation.?In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate[1]. |
| In vivo | mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection[2]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 4 mg/mL (15.49 mM), Sonication is recommended.
H2O : 40 mg/mL (154.9 mM), Sonication is recommended.
DMSO : 100 mg/mL (387.25 mM), Sonication is recommended.
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| Keywords | ribosome | protein | polysomes | phosphorylation | Nucleoside Antimetabolite/Analog | N1-Methylpseudouridine | N-1-Methylpseudouridine | N1Methylpseudouridine | N1 Methylpseudouridine | mRNA | Inhibitor | inhibit | HEK293T | GFP | expression | eIF2α | DNA/RNA Synthesis | cells |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Quinizarin | Adenine hemisulfate | Uridine | Rifaximin | Guanidine hydrochloride | Methotrexate disodium | Adenine | Carbazole | Thymidine | Docosanoic acid |
| Related Compound Libraries | Bioactive Compound Library | Bioactive Compounds Library Max | Nucleotide Compound Library |
United States
