| Name | N-Formyl-Met-Leu-Phe |
| Description | N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM). |
| Animal Research | ebrafish (Danio rerio) embryos were collected from spontaneous spawnings reared at 28.5 ℃. Three days postfertilization (～1 day after hatching), feeding of the commercial powder for baby fish was started. Zebrafish embryos were stimulated with fMLP from 8 to 48 h postfertilization (hpf), washed, and raised at 28.5 ℃. At 5 days postfertilization (dpf), 20 zebrafish were stained with Alizarin Red for examination of the effect of fMLP on bone formation. Zebrafish were fixed in 4% paraformaldehyde for 12–18 h. Following fixation, fish were depigmented overnight in 1% KOH containing 3% H2O2 and immersed for 2 h in 0.5% KOH containing 0.05% Alizarin Red, pH 4.2. Alizarin-stained fish were then cleared again in 1% KOH and observed under a microscope[2]. |
| In vitro | N-Formyl-Met-Leu-Phe receptor is associated with a Ni GTP regulatory protein, and N-Formyl-Met-Leu-Phe complex is important in the control of several neutrophil functions[1]. |
| In vivo | N-Formyl-Met-Leu-Phe(fMLP) promoted bone formation in zebrafish and rabbits, suggesting its physiological relevance in vivo[2]. |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 82.5 mg/mL (188.55 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (7.54 mM), Sonication is recommended.
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| Keywords | Tumor Necrosis Factor Receptor | TNFR | TNF Receptor | TNF | N-Formyl-Met-Leu-Phe | NFormylMetLeuPhe | N Formyl Met Leu Phe | Inhibitor | inhibit |
| Inhibitors Related | Acetylcysteine | Taxifolin | Acefylline | Glucosamine | Thalidomide | Apremilast | Pomalidomide | Bergenin | Lenalidomide | Fisetin | Linalool | CPI1189 |
| Related Compound Libraries | Apoptosis Compound Library | NF-κB Signaling Compound Library | Bioactive Compound Library | Anti-Alzheimer's Disease Compound Library | Cytokine Inhibitor Library | Membrane Protein-targeted Compound Library | HIF-1 Signaling Pathway Compound Library | Peptide Compound Library | Angiogenesis related Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max | Human Metabolite Library |
United States
