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ChemicalBook Product Catalog N-(2-hydroxyethyl)-Naphthalimide

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N-(2-hydroxyethyl)-Naphthalimide NEW

$47.00 50mg

$67.00 100mg

Min. Order
Purity100.00%
Cas No5450-40-8
Supply Ability10g
Update time2025-09-18

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

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United States

United States

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Chemical Properties

Product Name	N-(2-hydroxyethyl)-Naphthalimide
CAS No	5450-40-8
EC-No
Min. Order
Purity	100.00%
Supply Ability	10g
Release date	2025/09/18

Product Introduction

Bioactivity

Name	N-(2-hydroxyethyl)-Naphthalimide
Description	N-(2-hydroxyethyl)-Naphthalimide is a fluorescent probe for nucleic acid detection whose fluorescence can be burst.
Animal Research	I. Application in nucleic acid detection 1. Material preparation (1) HEN probe: dissolved in an appropriate organic solvent (such as DMSO or ethanol), the recommended concentration is 1-10 µM. (2) Sample: extracted DNA/RNA, or single-stranded nucleic acid for in vitro synthesis (such as specific oligonucleotides). (3) Buffer: buffer suitable for nucleic acid reaction (such as Tris-HCl, pH 7.5). 2. Experimental steps (1) Establishment of fluorescence detection system: Prepare the reaction system, including HEN probe and target nucleic acid, and ensure uniform mixing. The binding of probe to nucleic acid can quench the fluorescence signal by forming a complex or changing the surrounding environment. (2) Fluorescence signal monitoring: Record the change of fluorescence intensity on a fluorescence spectrometer, the excitation wavelength is usually 350-400 nm, and the emission wavelength is 450-500 nm. Evaluate the nucleic acid concentration or reaction kinetics by the change of fluorescence signal. (3) Nucleic acid comparison experiment: Compare the target nucleic acid sequence with the non-specific nucleic acid sequence to verify the selectivity and specificity of the probe. c. Data analysis Use the change in fluorescence signal intensity (or quenching efficiency) to draw a fluorescence titration curve, and calculate the concentration of the target molecule by nonlinear fitting. 2. Fluorescence imaging Experimental steps: (1) Load the HEN probe into the cell culture medium and incubate with living cells. (2) Use a fluorescence microscope to observe the distribution of the probe in the cell nucleus or cytoplasm and record the fluorescence intensity. (3) For specific nucleic acid targets, control experiments can be performed by adding RNA/DNA enzymes to verify the binding specificity of the probe.
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 30 mg/mL (124.36 mM), Sonication is recommended. Ethanol : 1 mg/mL (4.15 mM), Sonication is recommended. DMF : 30 mg/mL (124.36 mM), Sonication is recommended. DMSO:PBS (pH 7.2) (1:1) : 0.5 mg/mL (2.07 mM), Sonication is recommended.
Keywords	N-(2-hydroxyethyl)-Naphthalimide \| N(2hydroxyethyl)Naphthalimide \| N (2 hydroxyethyl) Naphthalimide

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