Fish Metallothionein‑2 MT‑2 Product Description
Overview Fish Metallothionein‑2 (MT‑2) is a small, cysteine‑rich, metal‑binding protein involved in heavy metal detoxification, oxidative stress response, and zinc homeostasis. This product provides a reliable immunoassay format for sensitive and specific quantification of MT‑2 in fish samples, supporting research in environmental toxicology, aquaculture health, and comparative physiology.
Values are representative configurations commonly provided by commercial MT‑2 ELISA kits for fish12345.
Features and benefits
High sensitivity and specificity with low cross‑reactivity for accurate MT‑2 measurement across fish species.
Convenient sample types: suitable for serum/plasma with a minimal sample volume (50–100 μL).
Rapid protocol: total assay time approximately 1–5 hours, enabling high‑throughput screening.
Validated readout at 450 nm with optional dual‑wavelength reference at 630 nm for improved accuracy.
Stable performance with clear plate layout and included reagents for consistent results13.
Kit components and storage
Pre‑coated microplate (96/48 wells), standards, sample diluent, detection antibody (biotinylated), enzyme conjugate (HRP‑avidin), TMB substrate, stop solution, and wash buffer.
Store kit components at 2–8°C in a dry, dark place; bring plates to room temperature before use.
Follow the provided instructions for reconstitution, plate sealing, and washing procedures to ensure optimal performance3.
Intended use and notes
For research use only (RUO); not for diagnostic or clinical use.
Ensure samples are free of hemolysis, lipids, and contaminants; avoid repeated freeze–thaw cycles.
Adhere to the recommended incubation times and temperatures; include appropriate controls for reliable quantification.
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