| Name | MPI-0479605 |
| Description | MPI-0479605 is an ATP competitive and selective inhibitor. |
| Cell Research | Cells are treated for 3 or 7 days with various concentrations of MPI-0479605 and the GI50 is determined. Cell viability is measured with CellTiter-Glo. (Only for Reference) |
| Kinase Assay | In Vitro Kinase Assays and Selectivity Screening : To measure Mps1 activity, 25 ng of recombinant, full-length enzyme is incubated in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 0.01% Triton X-100 and 5 μM Myelin basic protein (MBP)) containing vehicle (DMSO alone) or inhibitors. Forty μM ATP (2xKm) is added with 1 μCi [γ-33P]ATP and the reaction is incubated at room temperature for 45 minutes. Reactions are terminated with 3% phosphoric acid and transferred to P81 filter plates. Samples are washed in 1% phosphoric acid and 33P radioactivity is measured on a TopCount scintillation reader. In-house kinase assays are all carried out at 2xKm ATP concentrations. MPI-0479605 (500 nM) is also screened against a larger kinase panel. |
| In vitro | In a panel of tumor cell lines, MPI-0479605 caused a significant reduction in cell viability with a GI50 ranging from 30 to 100 nM. In addition, MPI-0479605 induced cell growth arrest and ultimately cell death through apoptosis or mitotic damage. chromosome segregation defects and alloploidy. |
| In vivo | In a panel of tumor cell lines, MPI-0479605 caused a significant reduction in cell viability with a GI50 ranging from 30 to 100 nM. In addition, MPI-0479605 induced cell growth arrest and ultimately cell death through apoptosis or mitotic damage. chromosome segregation defects and alloploidy. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 1 mg/ml, Sonication is recommended.
Ethanol : 2 mg/mL (4.9 mM), Heating is recommended.
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| Keywords | Apoptosis | Monopolar spindle 1 | MPI-0479605 | Inhibitor | MPI 0479605 | MPI0479605 | inhibit | Mps1 |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Acetylcysteine | Kaempferol | Myricetin | Sodium 4-phenylbutyrate | L-Ascorbic acid | Dextran sulfate sodium salt (MW 4500-5500) | Metronidazole | Sorafenib | Tributyrin | Lidocaine hydrochloride |
| Related Compound Libraries | Apoptosis Compound Library | Target-Focused Phenotypic Screening Library | Bioactive Compound Library | Kinase Inhibitor Library | Inhibitor Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Preclinical Compound Library | Anti-Cancer Compound Library | Anti-Cancer Active Compound Library |
United States
