| Name | Micheliolide |
| Description | Micheliolide(MCL) is a sesquiterpene lactone which inhibits various inflammatory response. |
| Cell Research | BV2 cells were pretreated with the indicated concentrations of Micheliolide?1 h prior to stimulation with LPS (1 μg/ml) for 6 h and total RNA was extracted using RaPure Total?RNA Kit.Reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit.?Real-time PCR analysis was performed using SYBR Select Master Mix ?in conjunction with an ABI Prism 7500 Sequence Detection System with the expression of β-actin as the internal control.?The data were analyzed using the ΔΔCT method.?The primers for iNOS, COX-2, TNF-α, IL-1β, IL-6 and β-actin were described as below. |
| In vitro | Treatment of BV2 cells with Micheliolide(MCL) significantly repressed LPS-stimulated nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, as well as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) induction.?MCL also attenuated mRNA levels of multiple pro-inflammatory cytokines and mediators such as iNOS, COX-2, TNF-α, IL-6 and IL-1β.?Mechanistic studies revealed that MCL suppressed LPS-stimulated the activation of IκBα/NF-κB pathway and Akt pathway.?Moreover, MCL inhibited LPS-induced the activition of c-Jun N-terminal kinase (JNK), p38 MAPK kinase, and extracellular signal-regulated kinases 1/2 (ERK1/2).?Meanwhile, MCL markedly promoted antioxidant protein heme oxygenase-1 (HO-1) expression by enhancing NF-E2-related factor 2 (Nrf2) activity.?Together, ?MCL may serve as a neuroprotective agent in neuroinflammation-related neurodegenerative disorders. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 49 mg/mL (197.32 mM)
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| Keywords | Micheliolide | Nuclear factor-κB | NF-κB | inhibit | Nuclear factor-kappaB | Inhibitor |
| Inhibitors Related | Bismuth Subsalicylate | L-NAME hydrochloride | Ibuprofen | Acetaminophen | Diclofenac sodium | Glucosamine | Diclofenac Potassium | Lidocaine hydrochloride | Indole-3-carbinol | Paradol | Diallyl disulfide | Salicylic acid |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Bioactive Compound Library | Traditional Chinese Medicine Monomer Library | Pain-Related Compound Library | Selected Plant-Sourced Compound Library | Natural Product Library | Inhibitor Library | Bioactive Compounds Library Max | TGF-beta/Smad Compound Library |
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