名称 | Mdivi-1 |
描述 | Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy. |
细胞实验 | Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system. |
激酶实验 | All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity. |
体外活性 | In the ganglion cell layer (GCL), Mdivi-1 exhibits strong immunoreactivity. Twelve hours following ischemic induction, Mdivi-1 significantly increases protein expression within the GCL. It notably reduces the expression of GFAP protein without altering Drp1 protein expression. In the normal murine retina, Mdivi-1 primarily localizes to the inner plexiform layer, ganglion cell layer, outer plexiform layer, and inner nuclear layer. Early in the development of the ischemic murine retina, there is a marked increase in the protein expression of Mdivi-1 and glial fibrillary acidic protein (GFAP). Mdivi-1 inhibits apoptosis in the ischemic retina and significantly increases the survival rate of retinal ganglion cells (RGC) two weeks post-ischemia. |
体内活性 | Mdivi-1 inhibits the ATPase activity and self-assembly of Dnm1 by inducing a conformational change (IC50<10 μM). It effectively suppresses C8-Bid and STS-induced mitochondrial outer membrane permeabilization (MOMP) in HeLa cells and extracellular mouse liver mitochondria. Mdivi-1 blocks the divisionof Dynamin-related GTPases, yeast Dnm1, and human Drp1, facilitating efficient and reversible mitochondrial fusion into a net-like structure. Intracellularly, it prevents apoptosis by inhibiting mitochondrial outer membrane permeabilization. Mdivi-1 represents a potential therapeutic class for treating stroke, myocardial infarction, and neurodegenerative diseases. |
存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
溶解度 | DMSO : 50 mg/mL (141.55 mM) 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.53 mg/mL (9.99 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
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关键字 | Mitochondrial Autophagy | Mitochondrial division inhibitor1 | Inhibitor | inhibit | Mdivi-1 | Autophagy | Mitophagy | Mdivi 1 | Mdivi1 | Mitochondrial division inhibitor-1 | Dynamin | Apoptosis |
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