| 名称 | MCB-613 |
| 描述 | MCB-613 is an effective steroid receptor coactivator (SRC) stimulator. |
| 细胞实验 | Cells are seeded in 96-well plates and allowed to reach 60% to 70% confluence. After indicated compound treatments, relative numbers of viable cells are measured by MTS assay using the Cell Titer 96 Aqueous One Solution Cell Proliferation Assay.(Only for Reference) |
| 激酶实验 | Luciferase assays: After various compound treatments, cells are lysed in luciferase lysis buffer and assayed for luciferase activity using the ONE-Glo luciferase assay system. All luciferase activities are normalized to protein concentration determined by Bradford assay. |
| 体外活性 | MCB-613 selectively and reversibly binds to the RID of SRC-3, and selectively kills cancer cells including MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells, without toxicity to mouse primary hepatocytes and mouse embryonic fibroblasts (MEFs). MCB-613 also increases SRCs' interactions with other coactivators and markedly induces ER stress coupled to the generation of reactive oxygen species (ROS). [1] |
| 体内活性 | In an MCF-7 breast cancer mouse xenograft model, MCB-613 (20 mg/kg, i.p.) significantly and dramatically inhibits the growth of the tumor while causing no obvious animal toxicity and body weight less. [1] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 56 mg/mL (184 mM)
DMSO : 56 mg/mL (184 mM)
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| 关键字 | inhibit | Inhibitor | MCB613 | MCB-613 |
| 相关产品 | Nintedanib | Vemurafenib | SI-2 hydrochloride | Staurosporine | Tandutinib | Bosutinib hydrate | Ibrutinib | Dasatinib | Ponatinib | Pelitinib | Tofacitinib | Masitinib |
| 相关库 | 抗胰腺癌化合物库 | 表型筛选靶点鉴定库 | 经典已知活性库 | 内质网应激化合物库 | 酪氨酸激酶分子库 | 激酶抑制剂库 | 抗癌细胞代谢库 | PPI抑制剂库 | 已知活性化合物库 | 抗癌活性化合物库 |
United States
