| Name | L-NAME hydrochloride |
| Description | L-NAME hydrochloride (L-NAME HCl), a cell-permeable NO synthase inhibitor, displays Ki values of 4.4 μM, 15 nM, and 39 nM, for iNOS (mouse) , nNOS (bovine) , and eNOS (human), respectively. |
| Cell Research | rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days. (Only for Reference) |
| Kinase Assay | Enzyme Assay: The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated. |
| In vivo | L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2] |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 50 mg/mL (185.4 mM), Sonication is recommended.
H2O : 27 mg/mL (100.11 mM), Sonication is recommended.
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| Keywords | NOSynthase | NOS | NO Synthase | nNOS | Nitric oxide synthases | NG-Nitroarginine methyl ester Hydrochloride | NG-Nitroarginine methyl ester | L-NAME Hydrochloride | LNAME hydrochloride | L-NAME | L NAME hydrochloride | iNOS | Inhibitor | inhibit | eNOS |
| Inhibitors Related | Aminoguanidine hydrochloride | Chondroitin sulfate | Mifepristone | 2,4-Diamino-6-hydroxypyrimidine | Dexamethasone acetate | S-Methylisothiourea sulfate | Nitroarginine | Ethyl cinnamate | Methylene Blue | Methylene Blue trihydrate | Diallyl disulfide | L-Arginine hydrochloride |
| Related Compound Libraries | Nonsteroidal Anti-Inflammatory Compound Library | Bioactive Compound Library | Membrane Protein-targeted Compound Library | Drug Repurposing Compound Library | Inhibitor Library | Neuroprotective Compound Library | NO PAINS Compound Library | Anti-Aging Compound Library | Immunology/Inflammation Compound Library | Clinical Compound Library | Bioactive Compounds Library Max |
United States
