| Name | KKL-35 |
| Description | KKL-35 is an inhibitor of trans-translation (IC50: 0.9 μM) with broad-spectrum antibiotic activity. |
| Cell Research | Overnight cultures of S. flexneri 2a 2457T, B. anthracis, M. smegmatis, JW5503, and NSR253 were grown in lysogeny broth (LB). Cultures were diluted to a final inoculum of ～5 × 10^5 cfu/mL in 96-well microtiter plates, compounds were added at appropriate concentrations, and growth was observed after 24-h incubation at 37 °C. The MIC was determined by the concentration of the compound in the last well that showed no bacterial growth. For MBC assays, 10 μL from wells containing the MIC, 2× MIC, and 4× MIC of each compound was diluted 10-fold, spotted on LB plates, and grown overnight at 37 °C. An inhibitor was scored as bactericidal if it resulted in 1,000-fold reduction in colony-forming units per milliliter from the original inoculum [1]. |
| In vitro | KKL-35 inhibited tagging of DHFR-ns. Dose-response experiments showed that KKL-35 inhibited tagging with an IC50 = 0.9 μM. KKL-35 prevented the growth of WT S. flexneri with a minimum inhibitory concentration (MIC) of 6 μM, and the addition of KKL-35 to a growing culture of S. flexneri stops growth [1]. Consistent with the essential nature of trans-translation in L. pneumophila, KKL-35 inhibited the growth of all tested strains at submicromolar concentrations. KKL-35 remained equally active against L. pneumophila mutants that have evolved resistance to macrolides. KKL-35 inhibited the multiplication of L. pneumophila in human macrophages at several stages of infection [2]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 8.33 mg/mL (26.23 mM), Sonication is recommended.
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| Keywords | Bacterial | Inhibitor | KKL-35 | KKL 35 | inhibit | KKL35 |
| Inhibitors Related | Neomycin sulfate | Dehydroacetic acid sodium | Ampicillin sodium | Methyl anthranilate | Doxycycline (hyclate) | Kanamycin sulfate | G-418 disulfate | Sulfamethoxazole sodium | Metronidazole | Doxycycline | Dimethyl sulfoxide | Crystal Violet |
| Related Compound Libraries | Bioactive Compound Library | Drug-induced Liver Injury (DILI) Compound Library | Antibiotics Library | Inhibitor Library | NO PAINS Compound Library | Anti-Bacterial Compound Library | Bioactive Compounds Library Max | Anti-Infection Compound Library |
United States
