| Name | INH1 |
| Description | INH1 (IBT13131) is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction. |
| Cell Research | Standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays with a 3-d drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH1 in cultured cells. Triplicate sets are measured and compiled for final data presentation.(Only for Reference) |
| Kinase Assay | Binding assays: Surface plasma resonance (SPR) assays are performed at 22.5°C in HBSD buffer [10 mmol/L HEPES, 150 mmol/L NaCl, 0.1% DMSO (pH 7.5)] on Biacore 3000. 6×His-Hec1 and GST-Nek2 are purified. NTA sensor chip or glutathione-modified CM5 chip are used to capture His-Hec1 and GST-Nek2, respectively. The capture level is about 140 to 180 resonance units (RU) at the flow rate of 5 μL/min. For the binding assay, chips are sequentially treated with compounds (1 or 20 μmol/L) and then proteins (50 μg/mL). Retained RUs are recorded and processed (triplicate experiments). |
| In vitro | Intraperitoneal injection of 100 mg/kg INH1 inhibits the growth of mammary tumors in mice with MDA-MB-468 human breast cancer xenografts. |
| In vivo | INH1 effectively inhibits the proliferation of human breast cancer cells with a GI50 of 10-21 μM. Additionally, INH1 induces cell-killing activity by disrupting the spindle checkpoint-regulated Hec1/Nek2 pathway. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 30.8 mg/mL (99.87 mM), Sonication is recommended.
Ethanol : 3.1 mg/mL (10.05 mM), Sonication is recommended.
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| Keywords | MicrotubuleAssociated | Microtubule Associated | Inhibitor | inhibit | INH-1 | INH1 | INH 1 | IBT-13131 | IBT 13131 | Hec1 | Apoptosis |
| Inhibitors Related | Stavudine | Cysteamine hydrochloride | Sodium 4-phenylbutyrate | Metronidazole | Citric Acid Triammonium | Tributyrin | L-Methionine | Sodium citrate | Cystamine dihydrochloride | L-Ascorbic acid sodium salt | Alginic acid | Dextran sulfate sodium salt (MW 5000) |
| Related Compound Libraries | Apoptosis Compound Library | Bioactive Compound Library | Microtubule-Targeted Compound Library | Inhibitor Library | NO PAINS Compound Library | PPI Inhibitor Library | Bioactive Compounds Library Max | Cytoskeletal Signaling Pathway Compound Library | Anti-Cancer Compound Library | Anti-Cancer Active Compound Library |
United States
