| 名称 | Hemin |
| 描述 | Hemin (Hemin chloride) is a chlorinated iron-containing porphyrin, a heme oxygenase (HO)-1 inducer. Hemin has therapeutic activity in porphyrias by reducing heme deficiency in patients, thereby inhibiting δ-aminolevulinic acid synthetase activity through biochemical feedback. |
| 细胞实验 | In vitro effects of various statins and hemin, a heme oxygenase inducer, on cell proliferation were evaluated in PA-TU-8902, MiaPaCa-2 and BxPC-3 human pancreatic cancer cell lines. The effect of statins on heme oxygenase activity was assessed and heme oxygenase-silenced cells were used for pancreatic cancer cell proliferation studies. Cell death rate and reactive oxygen species production were measured in PA-TU-8902 cells, followed by evaluation of the effect of cerivastatin on GFP-K-Ras trafficking and expression of markers of invasiveness, osteopontin (SPP1) and SOX2[1]. |
| 体外活性 | METHODS: Pancreatic cancer cells PA-TU-8902, BxPC-3 and MiaPaCa-2 were treated with Hemin (30 µM) for 48 h. Cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay.
RESULTS: Hemin had a significant effect on cell proliferation of PA-TU-8902, BxPC-3 and MiaPaCa-2 cell lines, decreasing cell proliferation to 62±5%, 51±3% and 38±8%, respectively. [1]
METHODS: Astrocyte cultures were treated with Hemin (25 µM) for 12-24 h. Iron content was measured using colorimetric method.
RESULTS: Iron accumulation occurred in cultured astrocytes after incubation with Hemin. [2] |
| 体内活性 | METHODS: To investigate the effects on renal injury, Hemin (100 µmol/kg) was administered intraperitoneally to BABL/c mice with renal ischemia-reperfusion.
RESULTS: Hemin pretreatment promoted ERK1/2 phosphorylation and enhanced tubular recovery, thereby preventing further kidney injury. [3]
METHODS: To investigate the effects on insulin resistance, Hemin (50 µmol/kg) was administered intraperitoneally to C57BL/6 mice on a high-fat diet once daily for four weeks.
RESULTS: Hemin prevented the development of high-fat diet-induced insulin resistance by increasing insulin sensitivity in skeletal muscle. [4] |
| 存储条件 | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | 0.05%DMSO+99.95% 0.1 mM NaoH : 0.33 mg/mL (50μM), Sonification is recommended.
DMSO : < 1 mg/ml
0.05%DMSO+99.95%PBS : 0.33 mg/mL (50μM)
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| 关键字 | Autophagy | Mitophagy | inhibit | Ferroptosis | Hemin Chloride | Inhibitor | Hemin | Mitochondrial Autophagy |
| 相关库 | 抗癌天然产物库 | 经典已知活性库 | 抗癌临床化合物库 | 药物功能重定位化合物库 | 天然产物库 | 抗癌上市药物库 | 高通量筛选天然产物库 | 抗衰老化合物库 | 已知活性化合物库 | 抗癌药物库 |
United States
