| Name | Flutamide |
| Description | Flutamide (SCH 13521) is an antiandrogen with about the same potency as cyproterone in rodent and canine species. |
| Cell Research | Effect of flutamide on the growth of an androgen-sensitive clone (SEM-l) of mouse mammary carcinoma Shionogi cells in culture. The cells are incubated up to 40 days in medium (MEM + 2% dextran-coated charcoal extracted fetal calf serum) containing the compounds at a concentration of 1 μM. Media are changed every second day.(Only for Reference) |
| Kinase Assay | Androgen Receptor Assay: Aliquots of 100 μl cytosol are incubated at 0-4°C for 18 h with 100 μl of the indicated saturating concentration of [3H]T in the presence or absence of increasing concentrations of nonlabeled T, DHT, flutamide (FLU) or flutamide-OH (FLU-OH). At the end of the incubation, free and bound T are separated by the addition of 200 μl dextran-coated charcoal (1 % charcoal, 0.1% dextran T-70, 0.1% gelatin, 1.5 mM EDTA and 50 mM Tris (pH 7.4)) for 15 min before centrifugation at 2300 × g for another 15 min at 0-4°C. Aliquots (350 μl) of the supernatant are transferred to scintillation vials with 10 ml of an aqueous counting solution before counting in a Beckman LS 330 counter. |
| In vitro | Flutamide significantly reduced the prostate weight in rats from 319 mg to 245 mg. Co-administration of Flutamide with a luteinizing hormone-releasing hormone (LHRH) agonist compounded side effects, further decreasing prostate weight to 101 mg and markedly diminishing prostatic ornithine decarboxylase (ODC) activity. |
| In vivo | The concurrent use of Flutamide and leuprorelin can be employed in the treatment of prostate cancer. The active metabolite of Flutamide, Flutamide-OH, binds directly to the androgen receptors in the anterior pituitary of rats (Ki=55 nM). Flutamide does not affect the proliferation of androgen-sensitive clones in the Shionogi SC-115 mouse mammary carcinoma cells when cultured, displaying only antiandrogenic effects without any androgenic activity. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 55 mg/mL (199.12 mM)
Ethanol : 51 mg/mL (184.6 mM)
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| Keywords | Inhibitor | SCH-13521 | inhibit | SCH13521 | Androgen Receptor | Flutamide |
| Inhibitors Related | Dehydroisoandrosterone 3-acetate | Bicalutamide | S-23 | Bavdegalutamide | Enzalutamide | Adrenosterone | Allura Red AC | SK33 | Sunset Yellow FCF | Ostarine |
| Related Compound Libraries | Bioactive Compound Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | Endocrinology-Hormone Compound Library | FDA-Approved Drug Library | Anti-Cancer Approved Drug Library | Orally Active Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library | Anti-Cancer Drug Library |
United States
