| Name | FH1 |
| Description | FH1 (NSC-12407) (BRD-K4477), a small molecule, enhances the functions of hepatocytes and promotes differentiation hepatocytes derived by iPS. |
| Kinase Assay | Affinity determination: Binding affinities (Ki or IC50) of ABT-263 against different isoforms of Bcl-2 family are determined with competitive fluorescence polarization assays. The following peptide probe/protein pairs are used: f-bad (1 nM) and Bcl-xL (6 nM), f-Bax (1 nM) and Bcl-2 (10 nM), f-Bax (1 nM) and Bcl-w (40 nM), f-Noxa (2 nM) and Mcl-1 (40 nM), and f-Bax (1 nM) and Bcl-2-A1 (15 nM). Binding affinities for Bcl-xL are also determined using a time-resolved fluorescence resonance energy transfer assay. Bcl-xL (1 nM, His tagged) is mixed with 200 nM f-Bak, 1 nM Tb-labeled anti-His antibody, and ABT-263 at room temperature for 30 min. Fluorescence is measured on an Envision plate reader using a 340/35 nm excitation filter and 520/525 (f-Bak) and 495/510 nm (Tb-labeled anti-His antibody) emission filters. |
| In vivo | Within hepatocytes, FH1(BRD-K4477) facilitates the differentiation of fully-integrated iHeps into a mature state, enhancing cellular functionality. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 4.62 mg/mL (16.36 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (3.54 mM), Sonication is recommended.
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| Keywords | NSC-12407 | NSC12407 | iPS-derived hepatocytes | iPSC | IPS | Inhibitor | inhibit | hRXR | hNTCP | hHNF4α | hALB | FH-1 | FH1 | FH 1 | BRD-K-4477 | BRD-K 4477 |
| Inhibitors Related | Furaneol | Epalrestat | 2-Chloro-1-(4-fluorobenzyl)benzimidazole | Dicoumarol | Lapisteride | Lidorestat | Alpha-Estradiol | Finasteride | AKR1C1-IN-1 | Kopexil | MitoBloCK-6 | Dutasteride |
| Related Compound Libraries | Bioactive Compound Library | NO PAINS Compound Library | Bioactive Compounds Library Max |
United States
