| Name | Ethopropazine Hydrochloride |
| Description | Ethopropazine Hydrochloride is the salt form of Ethopropazine (Profenamine), a phenothiazine derivative with anticholinergic, antihistamine, and anti-adrenergic effects used in the treatment of Parkinson's disease, and acts as an AChE inhibitor by affecting the PAS active site. |
| In vitro | Ellman’s method was used to assess the inhibitory activity of Ethopropazine Hydrochloride on cholinesterases, with human recombinant acetylcholinesterase (hAChE) and human plasma butyrylcholinesterase (hBChE) as target enzymes, and acetylthiocholine chloride (ATChCl) as the substrate. In a 96-well plate, enzyme solution, the chromogenic reagent DTNB, and different concentrations of Ethopropazine Hydrochloride (10 mM to 1 nM) were added sequentially. The reaction was initiated by adding 1 mM ATChCl, followed by incubation at 37°C for 5 minutes. Absorbance changes were measured at 412 nm. The Results showed that Ethopropazine Hydrochloride strongly inhibited hBChE (IC₅₀ = 1.6 μM), while its inhibitory effect on hAChE was weaker (IC₅₀ = 1020 μM), making it a highly efficient and selective BChE inhibitor [1]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : < 1 mg/mL (insoluble), Sonication is recommended.
DMSO : 20 mg/mL (57.32 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5.73 mM), Sonication is recommended.
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| Keywords | Parphezein | Parfezin | Pardisol | Lysivane | Ethopropazine Hydrochloride | Dibutil |
| Inhibitors Related | Adiphenine hydrochloride | Ethyl (triphenylphosphoranylidene) acetate | Urethane | L-Glutamic acid | Adenine | Mitotane | L-Glutamic acid monosodium salt | N,N-Dicyclohexylcarbodiimide | Dimethyl sulfoxide | Coumaran | Memantine hydrochloride | Memantine |
United States
