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ChemicalBook Product Catalog EthD-1

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EthD-1 NEW

$ 823.00 /1mg

Min. Order
Purity
Cas No61926-22-5
Supply Ability10g
Update time2026-01-04

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

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Chemical Properties

Product Name	EthD-1
CAS No	61926-22-5
EC-No
Min. Order
Purity
Supply Ability	10g
Release date	2026/01/04

Product Introduction

Bioactivity

Name	EthD-1
Description	EthD-1 (Ethidium homodimer) is a high-affinity fluorescent nucleic acid dye that emits red fluorescence upon binding to DNA or RNA, with excitation/emission wavelengths of 528/617 nm. EthD-1 is impermeable to cells and cannot be used in live cells.
In vitro	Instructions for the Use of EthD-1 (This protocol is for reference only and should be adjusted according to your specific experiment): 1. Stock Solution Preparation: Prepare a 2 mM EthD-1 stock solution using DMSO. Aliquot and store at -20°C or -80°C, protected from light. Avoid repeated freeze-thaw cycles. 2. Working Solution Preparation Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to a final concentration of 0.1–10 μM. Note: The optimal working concentration should be determined based on cell type and experimental requirements. The working solution must be prepared fresh before use. 3. Cell Preparation Suspension cells: Collect by centrifugation, wash twice with PBS (5 minutes each), and remove residual medium. Adherent cells: Discard the culture medium, detach cells using trypsin to create a single-cell suspension, centrifuge to remove supernatant, then wash twice with PBS (5 minutes each). Note: If not performing flow cytometry, adherent cells can be stained on coverslips. 4. Staining Incubation Add 1 mL of EthD-1 working solution and incubate at room temperature for 15–60 minutes, protected from light. Note: Prolonged incubation may lead to non-specific binding; it is recommended to optimize the incubation time via preliminary experiments. 5. Washing and Resuspension Centrifuge at 400 g for 3–4 minutes at 4°C to remove the staining solution. Wash cells twice with PBS (5 minutes each), then resuspend the cell pellet in 1 mL of serum-free culture medium or PBS. 6. Detect using fluorescence microscopy or flow cytometry.
Storage	keep away from direct sunlight \| store at -20°C \| Shipping with blue ice/Shipping at ambient temperature.
Keywords	Ethidium Homodimer-1 \| EthD-1 \| EthD 1
Inhibitors Related	Stavudine \| Aceglutamide \| Hemin \| Tamoxifen \| Cysteamine hydrochloride \| Sodium 4-phenylbutyrate \| Hydroxychloroquine \| Guanidine hydrochloride \| Paeonol \| Naringin \| Alginic acid \| Gefitinib

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