| Name | Ergolide |
| Description | Ergolide, a sesquiterpene lactone extracted from the dried flowers of Inula Britannica, effectively hinders the expression of inducible nitric oxide synthase and cyclo-oxygenase-2 in RAW 264.7 macrophages by deactivating NF-κB. |
| In vitro | Ergolide markedly decreased the production of prostaglandin E(2) (PGE(2)) in cell-free extract of LPS/IFN-gamma-stimulated RAW 264.7 macrophages in a concentration-dependent manner, without alteration of the catalytic activity of COX-2 itself.?Ergolide decreased the level of iNOS and COX-2 protein, and iNOS mRNA caused by stimulation of LPS/IFN-gamma in a concentration-dependent manner, as measured by Western blot and Northern blot analysis, respectively.?Ergolide inhibited nuclear factor-kappaB (NF-kappaB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-gamma.?This effect was accompanied by the parallel reduction of nuclear translocation of subunit p65 of NF-kappaB as well as IkappaB-alpha degradation.?In addition, these effects were completely blocked by treatment of cysteine, indicating that this inhibitory effect of ergolide could be mediated by alkylation of NF-kappaB itself or an upstream molecule of NF-kappaB.?Ergolide also directly inhibited the DNA-binding activity of active NF-kappaB in LPS/IFN-gamma-pretreated RAW 264.7 macrophages.?These results demonstrate that the suppression of NF-kappaB activation by ergolide might be attributed to the inhibition of nuclear translocation of NF-kappaB resulted from blockade of the degradation of IkappaB and the direct modification of active NF-kappaB, leading to the suppression of the expression of iNOS and COX-2,?which play important roles in inflammatory signalling pathway. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 50 mg/mL (163.21 mM), Sonication is recommended.
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| Keywords | Nuclear factor-kappaB | Nuclear factor-κB | Inhibitor | Ergolide | NF-κB | inhibit |
| Inhibitors Related | Sodium Houttuyfonate | Zingerone | Lidocaine | Diethylmaleate | Glucosamine | sodium lauroyl-α-hydroxyethyl sulfonate | Lidocaine hydrochloride | 5-Aminosalicylic Acid | Indole-3-carbinol | Diallyl disulfide | Caffeic Acid Phenethyl Ester | Sodium salicylate |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Terpene Natural Product Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Natural Product Library | Inhibitor Library | Natural Product Library for HTS | Anti-Aging Compound Library | Bioactive Compounds Library Max | Ancient Chinese Classical Formulas Compound Library | Food as Medicine Compound Library |
United States
