| Name | Epothilone A |
| Description | Epothilone A (Epo A) is a microtubule-stabilizing agent with EC0.01 of 2 μM. |
| Cell Research | Cell lines: KB3-1,KBV-1,Hela,and Hs578T cells. Concentrations: 0–2 μM. Method: cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips.After 24 hours,cells are treated with Epothilone A and are scored at regular intervals.For the cytotoxicity analysis,cells are counted and scored as trypan blue positive or negative. |
| Kinase Assay | Tubulin polymerization assay: Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone A is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope. |
| In vivo | Epothilone A induces microtubule polymerization, causing cell cycle arrest at the G2-M phase, and exhibits cytotoxicity, as well as induces apoptosis. It effectively inhibits the proliferation of HCT116 cells with an IC50 of 4.4 nM. Epothilone A possesses cytotoxicity against KB3-1, KBV-1, Hela, and Hs578T cells with IC50 values ranging from 13 nM to 160 nM. Additionally, Epothilone A competitively binds to microtubules against paclitaxel, with an IC50 of 2.3 μM. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (6.68 mM), Sonication is recommended.
H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 92 mg/mL (186.36 mM), Sonication is recommended.
DMSO : 92 mg/mL (186.36 mM), Sonication is recommended.
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| Keywords | Tubulin | MicrotubuleAssociated | Microtubule/Tubulin | Microtubule Associated | Inhibitor | inhibit | Epothilone A | Apoptosis |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Flubendazole | Cysteamine hydrochloride | Myricetin | Sodium 4-phenylbutyrate | L-Ascorbic acid | L-Glutamic acid | Tributyrin | L-Ascorbic acid sodium salt | Alginic acid | Dextran sulfate sodium salt (MW 5000) |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Apoptosis Compound Library | Bioactive Compound Library | Natural Product Library | Microtubule-Targeted Compound Library | Microbial Natural Product Library | Inhibitor Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
