| Name | Epirubicin hydrochloride |
| Description | Epirubicin hydrochloride (Pharmorubicin) is an adriamycin derivative, a topoisomerase (Topo) inhibitor, and a Forkhead box protein p3 (Foxp3) inhibitor. Epirubicin hydrochloride has antitumor activity. |
| Cell Research | Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37℃, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.(Only for Reference) |
| In vitro | METHODS: Neuroglioma cells U-87 and neuronal primary cultures were treated with Epirubicin hydrochloride (0.5-100 µM) for 48 h. Cell viability was measured by MTT assay.
RESULTS: Epirubicin significantly reduced the viability of rat U-87 cells in a concentration-dependent manner, with an IC50 of 6.3 µM. In contrast to U-87 glioma cells, rat normal neuronal cells were resistant to Epirubicin. [1]
METHODS: Human breast cancer cells MDA-MB-231 and SK-BR-3 were treated with Epirubicin hydrochloride (2-3 µM) for 48 h, and apoptosis was detected by Flow cytometry.
RESULTS: Epirubicin significantly increased sub-G cells in G2/M block. [2] |
| In vivo | METHODS: To test the antitumor activity in vivo, Epirubicin hydrochloride (7 mg/kg) was administered intravenously three times at four-day intervals to BALB/c nu/nu nude mice bearing MDA-MB-231 xenografts.
RESULTS: Epirubicin reduced tumor growth by half and resulted in a more than 10% weight loss. the IR value of Epirubicin at 7 mg/kg was 53.3%. [2] |
| Storage | store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | 5% DMSO+95% Saline : 2.9 mg/mL (5 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
DMSO : 42.86 mg/mL (73.89 mM)
Ethanol : 5.8 mg/mL (10 mM)
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| Keywords | 4'-Epidoxorubicin | Apoptosis | DNA/RNA Synthesis | Epirubicin hydrochloride | L-arabino | Epirubicin Hydrochloride | Inhibitor | Foxp3 | Epirubicin | 4'-Epidoxorubicin Hydrochloride | derivative | cell | inhibit | Topoisomerase |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Sodium 4-phenylbutyrate | L-Ascorbic acid | Dextran sulfate sodium salt (MW 4500-5500) | Guanidine hydrochloride | Metronidazole | Tributyrin | Thymidine |
| Related Compound Libraries | Bioactive Compound Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | Inhibitor Library | Anti-Cancer Approved Drug Library | FDA-Approved Drug Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library | Anti-Cancer Drug Library |
United States
