| 名称 | DMOG |
| 描述 | DMOG (Dimethyloxalylglycine), an antagonist of the α-ketoglutarate cofactor, is an inhibitor for HIF prolyl hydroxylase. |
| 细胞实验 | To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. (Only for Reference) |
| 体外活性 | DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3] |
| 体内活性 | DMOG inhibits endogenous HIF inactivation and induces angiogenesis in the ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG may be the cardioprotective mechanism of ischemic postconditioning in hyperlipidemic rats[4]. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | H2O : 17.54 mg/mL (100 mM), Sonication is recommended.
DMSO : 60 mg/mL (342.58 mM)
Ethanol : 35 mg/mL (199.84 mM)
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| 关键字 | Autophagy | diarrhoea | Hypoxia-inducible factors | stabilisation | Inhibitor | accmulation | HIF/HIF Prolyl-Hydroxylase | HIFs | inhibit | DMOG | HIF-PH | pro-angiogenic |
| 相关产品 | Hydroxychloroquine | Guanidine hydrochloride |
| 相关库 | 经典已知活性库 | 抗癌细胞代谢库 | 抑制剂库 | 抗衰老化合物库 | 已知活性化合物库 | 人代谢物化合物库 |
United States
