| Name | Dioscin |
| Description | Dioscin (Collettiside III) is a saponin with antitumor activities. |
| Kinase Assay | Rat liver cytosol is prepared from a separate piece of liver from rat. Assays are carried out in a total volume of 0.1 mL containing 10 mg of cytosolic protein, and components according to Rilling. All reaction components (except IPP) are mixed and kept on ice for 15 min. Reactions are initiated by the addition of [14C]IPP and incubation at 37°C. Reactions are stopped after 5 min by addition of 0.4 mL MeOH/HCl (4/1, by vol.) and the samples are incubated a further 15 min to hydrolyze the allylic pyrophosphates to petroleum ether-extractable products. Following addition of 0.5 mL water and 1 mL petroleum ether, 50% of the upper (petroleum ether-extractable) phase is taken for liquid scintillation analysis. Preliminary experiments indicated that the reaction is linear with time and protein under these conditions, and no more than 10% of the substrate is consumed. |
| In vitro | The chemical compound exhibits an LD50 value of greater than 300 mg/kg in mice, indicating the dosage at which half the population of test subjects succumbed to the compound's effects exceeded 300 mg/kg. |
| In vivo | Dioscin induces apoptosis by downregulating the activity of caspase-8 and upregulating the activity of caspase-9 in HeLa cells. It inhibits the growth of the MDA-MB-435, H14, HL60, and HeLa cell lines, with IC50 values of 2.6, 0.8, 7.5, and 4.5 μM, respectively. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 100 mg/mL (115.07 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 4 mg/mL (4.6 mM), Sonication is recommended.
Ethanol : 22 mg/mL (25.32 mM), Sonication is recommended.
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| Keywords | Inhibitor | inhibit | Dioscin | CCRIS-4123 | CCRIS4123 | Autophagy | Apoptosis |
| Inhibitors Related | Stavudine | Aceglutamide | Hemin | Tamoxifen | Urea | Cysteamine hydrochloride | Hydroxychloroquine | Metronidazole | Guanidine hydrochloride | Paeonol | Naringin | Alginic acid |
| Related Compound Libraries | Bioactive Compound Library | Traditional Chinese Medicine Monomer Library | Selected Plant-Sourced Compound Library | Anti-Fungal Compound Library | Drug Repurposing Compound Library | Miao medicine Compound Library | Anti-Cancer Approved Drug Library | Natural Product Library for HTS | Bitter Compound library | Bioactive Compounds Library Max | Ancient Chinese Classical Formulas Compound Library | Food as Medicine Compound Library |
United States
