| Name | Dihydrotanshinone I |
| Description | Dihydrotanshinone I (DHTS) is a natural compound extracted from Salvia miltiorrhiza Bunge used for treating of cardiovascular diseases. |
| Kinase Assay | Cells are treated with various concentrations of Dihydrotanshinone I (3.13-20 μM) for 48 h. For the activity assay, Ac-DEVD-AMC (1 μg/μL), Ac-IETD-AMC (1 μg/μL) or Ac-LEDH-AMC (1 μg/μL) and cell lysate are added into Protease Assay Buffer in 96-well plate. Reaction mixtures with lysis buffer are used as negative controls. Cells treated with DMSO (0.1%) are treated as vehicle control. The reaction mixtures are incubated for 1 h at 37°C. The AMC liberated from the substrates is measured using spectrofluorometer of Victor 2 plate reader with an excitation wavelength of 380 nm and an emission wavelength of 430 nm. |
| In vitro | Dihydrotestosterone (DHT, 10 nM) effectively reduces the expression of lectin-like ox-LDL receptor-1 (LOX-1) and NADPH oxidase 4 (NOX4), alongside diminishing reactive oxygen species (ROS) production, nuclear translocation of NF-κB, ox-LDL endocytosis, and monocyte adhesion in lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs)[1]. Additionally, Dihydrotanshinone I prompts caspase-dependent apoptosis in HCT116 cells, with this apoptosis being both concentration and ROS dependent. The presence of Z-VAD-fmk entirely prevents apoptosis, while pre-treatment with Z-LEHD-fmk significantly reduces it, and Z-IETD-fmk only achieves partial inhibition. Intriguingly, knocking down caspase-2 significantly enhances apoptosis induced by Dihydrotanshinone I[3]. |
| In vivo | In ApoE-/- mice fed with an atherogenic diet, DHT (10 and 25 mg kg-1) significantly attenuated atherosclerotic plaque formation, altered serum lipid profile, decreased oxidative stress and shrunk necrotic core areas. DHT dramatically inhibits the enhanced expression of LOX-1, NOX4, and NF-κB in aorta[1]. Dihydrotanshinone I (1, 2, 4 mg/kg) treatment can improve cardiac function, reduce infarct size, ameliorate the variations in myocardial zymogram and histopathological disorders, decrease 20-HETE generation, and regulate apoptosis-related protein in myocardial ischemia-reperfusion rats[2]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 3.85 mg/mL (13.82 mM), Sonication is recommended.
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| Keywords | SARS coronavirus | inhibit | SARS-CoV | Dihydrotanshinone I | Inhibitor |
| Inhibitors Related | Nirmatrelvir | EIDD-1931 | Remdesivir | Silymarin | Umifenovir hydrochloride | Hydroxychloroquine | Chloroquine phosphate | Tempol | Inosine | Lycopene | Dexamethasone | Ethyl cinnamate |
| Related Compound Libraries | Traditional Chinese Medicine Monomer Library | Bioactive Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Natural Product Library | Anti-Viral Compound Library | Anti-Aging Compound Library | Anti-infective Natural Product Library | Anti-virus Traditional Chinese Medicine Monomer Library | Bioactive Compounds Library Max |
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