Description of DEAE-Dextran from Wuhan Fortuna Chemical Co.,Ltd
Basic Information of DEAE-Dextran |
Product Name | DEAE-Dextran |
CAS NO. | 9015-73-0 |
Apperance | White powder |
Pacakge | 1kg/Foil Bag, 25kg/ Box/carton |
MOQ | 1kg |
Storage | Cool and dry place |
Shelf Life | 2 Years |
Brand | Fortunachem |
Specifications(COA) of DEAE-Dextran CAS 9015-73-0
Items | Specifications | Results |
Appearance | White to off white powder | White powder |
Loss on drying | ≤10% | Complies |
Determination of average molecular weight | 300,000-550,000 | Complies |
Nitrogen content | 2.0 ~ 5.0% | Complies |
Conclusion | The product comforms to standard |
Introduction of DEAE-Dextran is a cationic polymer derived from dextran (a glucose polysaccharide) modified with diethylaminoethyl (DEAE) groups, giving it a positive charge at neutral pH. Primarily used in molecular biology, it facilitates transfection by binding negatively charged DNA, forming complexes that adhere to cell membranes for uptake. It also serves as an anion exchanger in chromatography (e.g., DEAE-Sephadex) to purify proteins/nucleic acids. While largely replaced by lipofection or electroporation due to cytotoxicity at high doses, it remains cost-effective for specific cell types. Additional roles include nucleic acid precipitation and experimental drug/gene delivery. Optimal use requires pH control and minimized exposure to reduce cell damage.
Application of DEAE-Dextran is a chemically modified polysaccharide used primarily in molecular biology and biochemistry. Here's a structured overview:
Chemical Composition
Dextran Base: A polysaccharide composed of glucose units, known for its solubility and biocompatibility.
DEAE Modification: Diethylaminoethyl groups are attached, conferring a positive charge at physiological pH, making it a cationic polymer.
Key Applications
1. Transfection
Mechanism: Binds negatively charged DNA via electrostatic interactions, forming complexes that adhere to cell membranes. This facilitates cellular uptake, potentially through endocytosis or membrane disruption.
Use Case: Historically employed for introducing DNA into mammalian cells (e.g., COS, HeLa). Efficiency varies by cell type and requires optimization to balance efficacy and cytotoxicity.
Current Status: Largely supplanted by lipofection and electroporation but remains cost-effective for specific applications.
2. Chromatography
Ion Exchange Resin: DEAE-Dextran (e.g., DEAE-Sephadex) acts as a weak anion exchanger, separating proteins or nucleic acids based on charge. Used in purifying negatively charged biomolecules.
3. Other Uses
Nucleic Acid Precipitation: Can aggregate DNA/RNA via charge neutralization.
Drug/Gene Delivery: Explored as a carrier for charged therapeutic molecules, though toxicity limits in vivo use.
Considerations
Cytotoxicity: High concentrations can damage cells, restricting exposure time in transfection protocols.
pH Sensitivity: Charge density depends on pH, requiring buffered conditions for optimal performance.
Conclusion
DEAE-Dextran is a versatile tool in biotechnology, leveraging its cationic properties for transfection and chromatography. While newer methods have reduced its prevalence, it remains valuable in niche applications where cost and simplicity are prioritized.
FAQ From Wuhan Fortuna Chemical Co.,Ltd |
Q | Can I get a sample for free ? |
A | For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test. |
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Q | How to confirm the product quality before placing orders ? |
A | Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order. |
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Q | What is the payment you accept? |
A | For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us. |
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Q | What is your shipment way ? |
A | For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement. |
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Q | How long is your after-sales service? |
A | We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company. |
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Q | How do you treat quality complaint ? |
A | We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers. |
Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.
Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.
We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.
Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.
Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide DEAE-Dextran with good quality and best price, welcome to contact us to get COA/TDS/MSDS of DEAE-Dextran.
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