| Name | Colcemid |
| Description | Colcemid (NSC-3096) is a microtubule polymerization inhibitor that primarily targets tubulin, with an IC50 of 2.4 μM. Demecolcine interacts with tubulin dimers, induces antimitotic effects, and inhibits microtubule growth. Colcemid is a classic reagent used in karyotyping, banding techniques, and cell cycle synchronization. Colcemid can induce apoptosis and is used in studies of tumor and embryonic cloning. |
| In vitro | Methods: In a co-culture system of neonatal rat cardiomyocytes (CM) and myofibroblasts (MF), mitochondria were labeled with Ad-Mito-EGFP. After 4-hour treatment with Colcemid (4 μM), live cells were tracked in real-time using confocal microscopy to monitor mitochondrial movement within MNTs, and migration velocity was calculated.
Results: Colcemid treatment significantly reduced mitochondrial migration speed within MNTs but did not affect MNT formation. [1] |
| Storage | Keep away from moisture,Store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 55 mg/mL (148.08 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (5.38 mM), Sonication is recommended.
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| Keywords | tumor | NSC3096 | NSC 3096 | mitotic | MicrotubuleAssociated | Microtubule/Tubulin | Microtubule Associated | metaphase | Inhibitor | inhibit | derivative | Colcemid | cancer | apoptosis |
| Inhibitors Related | Stavudine | Aceglutamide | Urea | Tamoxifen | Cysteamine hydrochloride | Metronidazole | Citric Acid Triammonium | Formamide | Dimethyl phthalate | Alginic acid | Sodium Molybdate | Sildenafil citrate |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Bioactive Compound Library | Rare Natural Product Library | Alkaloid Natural Product Library | Selected Plant-Sourced Compound Library | Microtubule-Targeted Compound Library | Inhibitor Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library | Anti-Cancer Active Compound Library |
United States
