| Name | Cl-amidine hydrochloride |
| Description | Cl-amidine hydrochloride is an orally available PAD inhibitor that blocks histone 3 deimination and neutrophil extracellular trap formation and improves survival in septic mice. It induces apoptosis in cancer cells and induces miR-16 to cause cell cycle arrest. |
| In vitro | METHODS: Mouse primary splenocytes were treated with 1 μg/mL LPS and 10 μM Cl-amidine. Cell culture supernatants were collected 6 hours after treatment. TNF-α and pro-inflammatory properties of plasma and cell culture supernatants were determined by ELISA. Cytokine expression.
RESULTS Cl-amidine decreased the concentration of TNF-α in plasma. At the same time, Cl-amidine treatment could significantly reduce the concentrations of these pro-inflammatory cytokines (IL-1β, IL-6). [1]
METHODS: HT29 and TK6 cells were treated with Cl-amidine (0, 5, 10, 15, 25, 50 μg/mL, 24 hours) to detect whether Cl-amidine could induce apoptosis of HT29 and TK6 cells.
RESULTS Cl-amidine induced apoptosis of these cells in a dose-dependent manner. [2] |
| In vivo | METHODS: Cl-amidine (40 mg/kg) was administered intraperitoneally to mice 1 hour after cecal ligation and puncture (CLP) to observe the effect of Cl-amidine on protecting mice from sepsis-induced lethality. RESULTS Cl-amidine protected mice from sepsis-induced lethality, and Cl-amidine-treated CLP animals had a higher long-term survival rate. [1] |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 19.23 mg/mL (55.38 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (2.88 mM), Sonication is recommended.
H2O : 50 mg/mL (143.99 mM), Sonication is recommended.
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| Keywords | sepsis | Protein Arginine Deiminase | Peptidylarginine Deiminase | PAD4 | miRNA-16 | miRNA | miR-16 | microRNA-16 | MicroRNA | Inhibitor | inhibit | colitis | Cl-amidine Hydrochloride | Clamidine hydrochloride | Cl-amidine | Cl amidine hydrochloride | Apoptosis |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Flubendazole | Cysteamine hydrochloride | Myricetin | Sodium 4-phenylbutyrate | L-Ascorbic acid | L-Glutamic acid | Tributyrin | L-Ascorbic acid sodium salt | Alginic acid | Dextran sulfate sodium salt (MW 5000) |
| Related Compound Libraries | Apoptosis Compound Library | Bioactive Compound Library | Epigenetics Compound Library | Inhibitor Library | NO PAINS Compound Library | Orally Active Compound Library | Bioactive Compounds Library Max | Covalent Inhibitor Library | Anti-Cancer Compound Library | Anti-Cancer Active Compound Library |
United States
