| Name | Cholesterol |
| Description | Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia. |
| In vitro | METHODS: CD4+ T lymphocytes were incubated with 7-KC (17.5-70 µM) and Cholesterol-MβCD (17.5-70 µM) for 10 min, and T cell membrane order and disorder were assessed using di-4 ANEPPDHQ fluorescent dye.
RESULTS: After exposure to 7-KC, T cell membrane order was altered in a dose-dependent manner, with significant reconstitution of membrane order observed only in cells treated with 35 µM Cholesterol, while reconstitution with l7.5 µM Cholesterol induced minimal effects. [1]
METHODS: Human gastric cancer cells SNU601, SNU638 and SNU216 were treated with Cholesterol (25-100 µM) for 48 h and cell viability was measured using MTT Assay.
RESULTS: Cholesterol caused a dose-dependent decrease in cell viability in all three cell lines. [2] |
| In vivo | METHODS: To induce hypercholesterolemia, STD:ddY mice were fed a high cholesterol diet (1% cholesterol, 0.5% cholic acid, 0.5% olive oil and 93% standard mouse chow).
RESULTS: Cholesterol can be used to construct a mouse model of hypercholesterolemia. [3]
METHODS: To induce hyperlipidemia, CD-1 mice were fed a high cholesterol diet (2% cholesterol and 0.6% sodium deoxycholate).
RESULTS: Cholesterol can be used to construct a mouse model of hyperlipidemia. [4] |
| Storage | store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : < 1 mg/mL (insoluble)
DMSO : < 1mg/mL (insoluble)
Ethanol : 11.42 mg/mL (29.54 mM), Sonication is recommended.
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| Keywords | unesterified | transporters | SREBP2 | signaling | RORα | phosphorylation | MRP1 | IRS-1 | insulin | Inhibitor | inhibit | exocytic | EstrogenReceptor | Estrogen Receptor/ERR | Estrogen Receptor | ERRα | ERR | EndogenousMetabolite | Endogenous Metabolite | Diabetes | Cholesterol | AKT |
| Inhibitors Related | Sucrose | Neomycin sulfate | Aceglutamide | Emtricitabine | Levulinic acid | D(+)-Raffinose pentahydrate | Glycerol | Thymidine | Dimethyl sulfoxide | Gluconate Calcium | Sodium diacetate | BES |
United States
