| Name | Bufalin |
| Description | Bufalin is a natural product and a Na⁺/K⁺-ATPase inhibitor (IC50 ≈ 40–45 nM) with favorable cell permeability. By binding to α subunits such as ATP1A1, bufalin induces calcium overload and pyroptosis, and can be used for experimental research and therapeutic exploration in various cancers. |
| In vitro | Methods: Human lung cancer cells A549 and H1299 were incubated with 40 ng/ml Bufalin for 24 hours.
Results: Bufalin significantly inhibited cell viability, colony formation, and soft agar colony formation in a concentration-dependent manner.[1]
Methods: Human colon cancer HCT-116 and SW620 cells were incubated with gradient concentrations of Bufalin for 48 hours, respectively.
Results: Bufalin inhibited cell proliferation in a concentration-dependent manner, with IC₅₀ values of 12.823±1.792 nM and 26.303±2.498 nM, respectively.[2] |
| In vivo | Methods: A549 or H1299 cells were inoculated into nude mice. When tumor volume reached 220–250 mm³, Bufalin 1 mg/kg was administered by subcutaneous or intraperitoneal injection daily or every other day (with DMSO as the vehicle) for 25 consecutive days.
Results: Tumor volume and weight in the Bufalin group were significantly lower than those in the control group. [1] |
| Storage | Keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 4 mg/mL (10.35 mM), Sonication is recommended.
DMSO : 100 mg/mL (258.72 mM), Sonication is recommended.
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| Keywords | Sodium potassium pump | Na+/K+-ATPase | Na+/K+ ATPase | Inhibitor | inhibit | Bufalin |
| Inhibitors Related | Tirofiban hydrochloride monohydrate | Sodium oleate | Permethrin | Tolbutamide | trans-Aconitic acid | Phlorizin | Chlorpropamide | 2,3-Butanedione 2-Monoxime | Revaprazan hydrochloride | Oleic acid | Cyclocreatine | Esomeprazole Sodium |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Traditional Chinese Medicine Monomer Library | Bioactive Compound Library | Endoplasmic Reticulum Stress Compound Library | Membrane Protein-targeted Compound Library | Anti-Inflammatory Traditional Chinese Medicine Compound Library | Natural Product Library | Inhibitor Library | Natural Product Library for HTS | Immunology/Inflammation Compound Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library |
United States
