| 名称 | Bergapten |
| 描述 | Bergapten (5-Methoxypsoralen), a psoralen, inhibits cell replication. |
| 细胞实验 | 5-Methoxypsoralen (5-MOP) is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO <0.1%) before use[1]. The human colon adenocarcinoma cell line (COLO 205, from a 70-year-old male Caucasian) sre placed into 75-cm2 tissue culture flasks and grown in RPMI1640 medium, supplemented with 10% fetal bovine serum, containing penicillin and streptomycin (100 Ig/mL) and 1 mM glutamine, at 37°C in a humidified atmosphere of 5% CO2 and 95% O2. The human stomach adenocarcinoma cell line are placed into 75-cm2 tissue culture flasks and grown in RPMI 1640 medium, supplemented with 10% fetal bovine serum, containing penicillin and streptomycin (100 Ig/mL) and 1 mM glutamine, at 37°C in a humidified atmosphere of 5% CO2 and 95% O2. SC-M1 and COLO 205 cells are treated with different concentrations of 5-MOP (0.05, 0.5, 5, 10, 25 and 50 mM) and incubated for 72 h for the dose-effect study of 5-MOP on NAT activity. To determine the time-course effect of 0.5 mM 5-MOP on NAT activity, the cells are incubated at 37AC and harvested at 12, 24, 48 and 72 h, respectively. 5-MOP is dissolved in DMSO and the final concentration of vehicle is <0.1%. Only DMSO (solvent) is added for the control regimen[1]. |
| 体外活性 | In C57BL/6J mice induced with acetaminophen (APAP), Bergapten exhibits antioxidant activity and mitigates hepatotoxicity. |
| 体内活性 | In both tamoxifen-sensitive and -resistant breast cancer cells, Bergapten eliminates ERα and reprograms membrane signaling targeted at ERα, thereby enhancing the potential for cell mitosis. |
| 存储条件 | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | Ethanol : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 20 mg/mL (92.51 mM), Sonication is recommended.
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| 关键字 | Bergapten | inhibit | Inhibitor | Autophagy | CYPs | Cytochrome P450 |
| 相关产品 | Stavudine | Aceglutamide | Sodium 4-phenylbutyrate | Hydroxychloroquine | Guanidine hydrochloride | Taurine | Valproic Acid | Curcumin | Paeonol | Naringin | Salicylic acid | Gefitinib |
| 相关库 | 抗癌天然产物库 | 中药单体化合物库 | 植物来源化合物库 | 抗癌临床化合物库 | 中药抗炎分子库 | 抑制剂库 | 烟草单体化合物库 | 高通量筛选天然产物库 | 古代经典名方目录分子库 | 抗癌药物库 |
United States
