| Name | Bay 65-1942 hydrochloride |
| Description | Bay 65-1942 hydrochloride is an ATP-competitive and selective inhibitor of IKKβ. |
| In vitro | Administration of Bay 65-1942 before ischemia significantly reduces left ventricular infarct size compared to vehicle-treated animals, with observed reductions at all time points (prior to ischemia 42.7±4.1%, at reperfusion 42.7±7.5%, 2 hours of reperfusion 29.4±5.2%; each group P<0.05 vs. vehicle). Compared to sham animals, vehicle-treated animals show a significant increase in the infarct-to-area at risk (AAR) ratio (70.7±3.4 vs. 5.8±3.4%, P<0.05). Bay 65-1942 pretreatment (n=3) significantly lowers CK-MB levels compared to untreated animals prior to IR (14,170 ±3,219 units, P<0.05 vs. vehicle)[1]. |
| In vivo | AZD6244 and BAY 65-1942 demonstrate synergistic inhibition of cell viability at the dose combination (5 μM AZD6244+10 μM BAY 65-1942), which correlates with IC75 (CI?=?0.48±0.01). AZD6244 and BAY 65-1942 treatment induces 2- and 1.3-fold caspase 3/7 activation, respectively, compared to the DMSO-treated cells. Treatment with a combination of AZD6244 plus BAY 65-1942 leads to a 3.2-fold increase in caspase 3/7 activity[2]. Inhibitors of MEK (AZD6244) and IKK (BAY 65-1942) are used at their IC50 concentrations, as determined by a 48 hour MTS assay, which achieve sufficient inhibition of kinase activity. MYL-R cells are treated for 24 hours with AZD6244 (5 μM), BAY 65-1942 (10 μM), or a combination of these inhibitors at the same concentrations. Synergism is also indicated at the IC50 (CI?=?0.56±0.09) and IC90 (CI?=?0.46±0.02) dose combinations reported by the software (CI values are the mean of three independent experiments, ± standard deviation). |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (115.76 mM), Sonication is recommended.
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| Keywords | IκB | IKKβ | IKK | IkB/IKK | IkB | Bay 65-1942 Hydrochloride | Bay 651942 hydrochloride | Bay 65 1942 hydrochloride |
| Inhibitors Related | JTP 0819958 - HOIPIN-1 | Amlexanox | Malachite green oxalate | Rosmarinic acid | BAY 11-7082 | SC-514 | Resveratrol | Bardoxolone Methyl | GSK8612 | BX795 | CCCP | Allicin |
United States
