| Name | ADU-S100 disodium salt |
| Description | ADU-S100 (MIW815) disodium salt is an activator of stimulator of interferon genes (STING). |
| Cell Research | Cryopreserved hPBMCs are thawed and 1×10^6 cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO2. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer. |
| Animal Research | WT C57BL/6 mice are inoculated with 5×10^4 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10^4 B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm3 they received three IT doses of ADU-S100 at 5, 25, 50, or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17, and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C). |
| In vitro | ADU-S100 shows enhanced type I IFN production over CDA in THP-1 human monocytes. ADU-S100 induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. ADU-S100 is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). ADU-S100 induces significantly higher levels of IFN-α when compared to ML cGAMP. |
| In vivo | ADU-S100 demonstrates superior anti-tumor efficacy compared to the endogenous molecule ML cGAMP. Upon conducting a dose-response analysis in B16 tumor-bearing mice with the ADU-S100 compound, an optimal dose for maximal antitumor activity was identified. This optimal dose not only elicits heightened tumor antigen-specific CD8+ T cell responses but also significantly enhances long-term survival rates to 50%. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O : 300 mg/mL (408.44 mM), Sonication is recommended.
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| Keywords | STING | ML RR-S2 CDA disodium | ML RR-S2 CDA | MIW815 disodium | MIW-815 | MIW815 | MIW 815 | ADU-S-100 disodium salt | ADUS100 disodium salt | ADU-S100 disodium | ADU-S-100 | ADU-S100 | ADU-S 100 | ADU S100 disodium salt |
United States
