| Name | Acetylcholine iodide |
| Description | Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system. |
| Cell Research | The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.(Only for Reference) |
| In vitro | Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells[2]. |
| In vivo | Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury[2]. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 50 mg/mL (183.08 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (7.32 mM), Sonication is recommended.
H2O : 33 mg/mL (120.83 mM), Sonication is recommended.
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| Keywords | Inhibitor | inhibit | ACh | Acetylcholine Iodide | Acetylcholine iodide | Acetylcholine |
| Inhibitors Related | Sucrose | Aceglutamide | L-Pyroglutamic acid | Emtricitabine | Levulinic acid | D(+)-Raffinose pentahydrate | D-(+)-Trehalose dihydrate | Glycerol | Thymidine | N,N-Dicyclohexylcarbodiimide | Gluconate Calcium | Chitosan oligosaccharide |
| Related Compound Libraries | FDA-Approved & Pharmacopeia Drug Library | Bioactive Compound Library | Approved Drug Library | Natural Product Library | Drug Repurposing Compound Library | NO PAINS Compound Library | Natural Product Library for HTS | FDA-Approved Drug Library | RO5 Drug-like Natural Product Library | Bioactive Compounds Library Max |
United States
