| Name | Ac-DEVD-AMC |
| Description | Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 . |
| Cell Research | Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2]. |
| In vitro | The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2]. |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : ≤ 10 mg/mL (14.80 mM), Sonication is recommended.
H2O : < 0.1 mg/mL (insoluble)
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| Keywords | Inhibitor | inhibit | Caspase-3 | Caspase | Ac-DEVD-AMC | AcDEVDAMC | Ac DEVD AMC |
| Inhibitors Related | Taurodeoxycholic acid | Allethrin | Crustecdysone | SKLB-163 | Fenbufen | Cystamine | Taurochenodeoxycholic Acid | Tauroursodeoxycholate sodium | (Iso)-Z-VAD(OMe)-FMK | CIL62 | Tauroursodeoxycholate | PAC-1 |
| Related Compound Libraries | Apoptosis Compound Library | Bioactive Compound Library | Anti-Neurodegenerative Disease Compound Library | Membrane Protein-targeted Compound Library | Protease Inhibitor Library | Pyroptosis Compound Library | Peptide Compound Library | Bioactive Compounds Library Max | Anti-Cancer Compound Library |
United States
