| Name | Abemaciclib methanesulfonate |
| Description | Abemaciclib methanesulfonate (LY2835219) is a specific and effective inhibitor of CDK4(IC50=2 nM) and CDK6(IC50=10 nM). |
| Cell Research | LY2835219 is dissolved in DMSO to a 10 mM concentration.? Cells are seeded in a 96-well plate, allowed to adhere overnight, and treated with DMSO control (0.1% v/v) or the indicated compounds for 72 h. Cell viability and proliferation are determined using a Cell Counting Kit according to the manufacturer's instructions. The interaction between LY2835219 and mTOR inhibitor is determined using CompuSyn. Combination index (CI) values of 1 indicates and additive drug interaction, whereas a CI of < 1 is synergistic and a CI of > 1 is antagonistic. |
| Kinase Assay | Cells (5 × 103) are plated in 96 well plates. Cells are treated the next day for 24 to 48 hours and then assessed for caspase-3 activity by Caspase-Glo-3/7 Assay, as per manufacturer's instructions and a luminescence plate reader. |
| In vitro | In subcutaneous and intracranial glioma models (U87 mg), MsOH treatment results in LY2835219 inhibiting tumor growth in a dose-dependent manner. |
| In vivo | In retinoblastoma, LY2835219 inhibits cancer cell growth by specifically targeting and inhibiting CDK4 and CDK6, leading to cell cycle arrest at the G1 phase and suppression of DNA synthesis. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 1 mg/mL (1.66 mM), Sonication is recommended.
H2O : 45 mg/mL (74.66 mM), Sonication is recommended.
DMSO : 6.03 mg/mL (10 mM), Sonication is recommended.
Ethanol : 23 mg/mL (38.16 mM), Sonication is recommended.
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| Keywords | methanesulfonate | LY-2835219 Mesylate | LY2835219 Mesylate | LY-2835219 | LY 2835219 Mesylate | LY 2835219 | Inhibitor | inhibit | Cyclin dependent kinase | CDK6 | CDK4 | CDK | Abemaciclib methanesulfonate | abemaciclib Mesylate | Abemaciclib |
| Inhibitors Related | Ribociclib | Amantadine | 2-Chloropyrazine | Kojic acid | Ro-3306 | Olomoucine | Abemaciclib | Palbociclib | GW 441756 | Sodium Oxamate | Seliciclib | Dinaciclib |
| Related Compound Libraries | Failed Clinical Trials Compound Library | Bioactive Compound Library | Kinase Inhibitor Library | EMA Approved Drug Library | Anti-Cancer Clinical Compound Library | Drug Repurposing Compound Library | FDA-Approved Kinase Inhibitor Library | Anti-Cancer Approved Drug Library | Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Cancer Drug Library | Anti-Cancer Active Compound Library |
United States
