2'-deoxy-2'-fluoro-2'-C-methylcytidine

PHOSPHOENOLPYRUVIC ACID MONOPOTASSIUM SALT Basic information

Product Name:

PHOSPHOENOLPYRUVIC ACID MONOPOTASSIUM SALT

Synonyms:

2-Propenoic acid,2-(phosphonooxy)-, potassium salt (1:1);2-[PHOSPHONOOXY]-2-PROPENOIC ACID MONOPOTASSIUM SALT;potassium hydrogen 2-(phosphonatooxy)acrylate;PHOSPHO(ENOL)PYRUVATE MONOPOTASSIUM;PEP, potassium salt;Phosphoenolpyruvic Acid, Monopotassium Salt (unlabelled);Potassium Phosphoenol Pyruvate-2,3-13C2;Potassium Phosphoenol Pyruvate-2-13C

CAS:

MF:

C3H4KO6P

MW:

206.13

EINECS:

224-247-0

Product Categories:

Building Blocks;Chemical Synthesis;Organic Building Blocks;Organic Phosphates/Phosphites;Phosphorus Compounds;metabolite

Mol File:

4265-07-0.mol

 

PHOSPHOENOLPYRUVIC ACID MONOPOTASSIUM SALT Chemical Properties

Melting point 

175 °C (dec.)(lit.)

storage temp. 

−20°C

form 

solid

Water Solubility 

Soluble in water. Slightly soluble in methanol.

Sensitive 

Hygroscopic

BRN 

4603446

CAS DataBase Reference

4265-07-0(CAS DataBase Reference)

 

Safety Information

Safety Statements 

24/25

WGK Germany 

3

F 

3-10

TSCA 

Yes

HS Code 

29199000

MSDS Information

 

PHOSPHOENOLPYRUVIC ACID MONOPOTASSIUM SALT Usage And Synthesis

Chemical Properties

white to light yellow fine crystalline powder

Uses

Substrate for many kinase reactions.

Purification Methods

KPEP is purified via the monocyclohexylamine salt (see next entry). The salt (534mg) in H2O (10mL) is added to Dowex 50Wx4 H+ form (200-400 mesh, 2mL, H2O washed) and stirred gently for 30minutes and filtered. The resin is washed with H2O (6mL), and the combined solutions are adjusted to pH 7.4 with 3N KOH (~1.4mL) and the volume adjusted to 18.4mL with H2O to give a solution of 0.1M KPEP which can be lyophilised to a pure powder and is very good for enzyme work. It has been recrystallised from MeOH/Et2O. [Clark & Kirby Biochemical Preparations 11 103 1966, Wold & Ballou J Biol Chem 227 301 1957, Cherbuliez & Rabinowitz Helv Chim Acta 39 1461 1956, Beilstein 3 IV 977.] The triNa salt [5541-93-5] M 360.0, is purified as follows: the salt (1g) is dissolved in MeOH (40mL) and dry Et2O is added in excess. The white crystals are collected and dried over P2O5 at 20o. [Cramer & Voges Chem Ber 92 952 1959, Beilstein 3 IV 977.]

 

PHOSPHOENOLPYRUVIC ACID MONOPOTASSIUM SALT Preparation Products And Raw materials